短芒大麦草组织培养体系的建立

Establishment of Tissue Culture System for Hordeum brevisubulatum(Trin.)Link.

以短芒大麦草幼穗为外植体,建立了比较稳定高效的组织培养体系。研究结果表明,在含有3.0-4.0mg/L 2,4-D的MS＋300mg/L CH＋3%蔗糖＋0.7%琼脂（pH5.8）培养基上,短芒大麦草幼穗切段愈伤组织诱导率可达到80.0%以上;经MS＋300mg/LCH＋0.5mg/L 2,4-D＋3%蔗糖＋0.7%琼脂（pH5.8）培养基上继代培养后,其胚性愈伤组织在MS＋300mg/LCH＋0.5mg/L 2,4-D＋0.1mg/L 6-BA＋3%蔗糖＋0.7%琼脂（pH5.8）分化培养基上的分化率为62.9%;短芒大麦草组培苗在1/2MS＋NAA0.1mg/L＋蔗糖1%＋琼脂0.7%（pH5.8）培养基上生根后,移栽成活率可达到98.0%以上。

A stable tissue culture protocol was presented for efficient plant regeneration from callus induction of young inflorescence of Hordeum brevisubulatum（Trin.）Link.The result showed that the induction rate of primary callus from young inflorescence sections of Hordeum brevisubulatum（Trin.）Link could be above 80.0% on the callus induction medium contained MS basal salts and vitamins,3.0or4.0mg/L 2,4-D,300mg/L CH and 3% sucrose and solidified with 0.7% agar（pH5.8）.After the callus was subcultured on the subculture medium contained MS basal salts and vitamins,300mg/L CH,0.5mg/L 2,4-D and 3% sucrose and solidified with 0.7% agar（pH5.8）,the differentiation rates of embryogenic callus were62.9% on the differentiation medium contained MS basal salts and vitamins,300mg/L CH,0.5mg/L 2,4-D,0.1mg/L 6-BA and 3%sucrose and solidified with 0.7%agar（pH5.8）.The regenerants developed many strong roots readily on rooting medium contained 1/2MS,0.1mg/L NAA and 1% sucrose and solidified with 0.7% agar（pH5.8）,and the survival rate of regenerants were more than 98.0% after they were transplanted to soil.