体内光量子点可视化图像在脑肿瘤中的应用

In Vivo Optical Imaging Using Quantum Dots for the Application of Brain Tumors

恶性胶质瘤年发病率约为5/100,000。美国每年有超过14,000例的新发恶性脑胶质瘤患者。治疗主要以手术治疗为主,手术肿瘤的切除程度影响患者的预后。外科手术治疗脑肿瘤需要精确定位脑肿瘤组织在正常脑组织中的位置以便能够获得精确的组织活检和肿瘤的完全切除。量子点是稳定存在的,产生荧光的可视化半导体纳米晶体。静脉注射量子点伴随着网状内皮系统和巨噬细胞的隔离。巨噬细胞可渗入到肿瘤组织并且能够吞噬通过静脉注射的光量子来产生可视化的肿瘤标记。通过巨噬细胞介导,将光量子运输至肿瘤组织展现了一种新兴技术来标记术前肿瘤组织。由于肿瘤组织中的光量子可以被光学成像和光谱学工具来探测,因此在脑肿瘤组织活检和切除中可以为外科医生提供可视化得实时反馈。

The annual incidence of malignant glioma is approximately 5/100,000.There are more than 14,000 new malignant glioma patients each year in USA.The surgical management of brain tumors requires the precise localization of tumor tissues within normal brain parenchyma in order to achieve accurate diagnostic biopsy and complete surgical resection.Quantum dots are optical semiconductor nanocrystals that exhibit stable,bright fluorescence.The intravenous injection of quantum dots is accompanied by reticuloendothelial system and macrophage sequestration.Macrophages infiltrate brain tumors and phagocytize intravenously injected quantum dots,optically labeling the tumors.Macrophage-mediated delivery of quantum dots to brain tumors may represent a novel technique to label tumors preoperatively.Quantum dots within tumors may be detected with optical imaging and optical spectroscopy tools,providing the surgeon with real-time optical feedback during the resection and biopsy of brain tumors.