摘要
目的:探讨川芎嗪对急性髓性白血病KG-1a细胞表面标志物的影响。方法:通过体外细胞培养技术,利用中药有效成分单体干预细胞生长,运用MTT法及流式细胞术检测了经川芎嗪干预后的KG-1a细胞表面标志物CD34、CD33、CD123与CD7、CD56、CD44表达。结果:川芎嗪干预KG-1a细胞48小时后,细胞表面标志物CD34+CD33+、CD34+CD123+、CD33+CD123+表达率较对照组明显减少(P<0.05),但是对于CD34+表达率无明显作用,同时,川芎嗪干预KG-1a细胞48小时后,KG-1a细胞表面标志物CD7、CD56、CD44荧光强度与对照组比较明显降低(P<0.05),但是对KG-1a细胞表面标志物CD7、CD56、CD44表达率无明显影响,与对照组比较无统计学意义(P>0.05)。结论:川芎嗪逆转白血病耐药作用,除能够降低MDR、P-gp高表达外,能够降低白血病干细胞特异性表面标志物CD34+CD123+、CD34+CD33+、CD33+CD123+、CD7、CD56、CD44表达水平,从白血病干细胞水平逆转多药耐药。
Objective： To study the TMP on acute myelogenous leukemia KG-la cell surface markers. Methods： By in vitro cell culture techniques, using flow cytometry, the cell surface markers of CD34, CD33 and CD123 CD7, CD56, and CD44 expressions were examined after the TMP intervention ofKG - la cell. Results： TMP had no effect on the CD34＋ expression, and reduced the levels of ex- pressions CD34＋ CD123＋, CD34＋CD33＋, and CD33＋CD123＋ in KG-la cells （P〈0.05）. Meanwhile, TMP also decreased the fluorescence intensity of CD7, CD56, and CD44（P 〈 0.05 ）. Conclusion： TMP can reverse leukemia drug resistance, and reduce the expression of LSC surface markers including CD34＋CD123＋, CD34＋CD33＋, CD33＋CD123＋, CD7, CD56, and CD44.
出处
《现代生物医学进展》
CAS
2015年第5期844-848,共5页
Progress in Modern Biomedicine
基金
教育部高等学校博士点专项基金(20100013110008)
北京市教委共建科研基地项目(2012年北京中医药大学)
