江南牡丹茎段愈伤组织诱导与植株再生

Efficient Induction of Callus and Plant Regeneration From Paeonia suffruticosa Andr.

为探讨不同培养基、不同植物生长调节剂对江南牡丹茎段愈伤组织诱导及植株再生的影响,本研究以江南牡丹凤丹幼嫩茎段为材料,通过愈伤组织途径建立了植株再生体系。结果表明:改良MS培养基为牡丹茎段愈伤组织诱导的最佳培养基,愈伤组织最高诱导率为93.3%,最佳的培养基组合为改良MS+2,4-D0.2mg·L-1+6-BA2.0 mg·L-1+NAA0.3 mg·L-1。在愈伤组织分化过程中,加入KT0.2mg·L-1效果最好,有机物也能促进愈伤组织分化,其中加入水解酪蛋白300 mg·L-1效果最佳。牡丹茎段愈伤组织分化培养基为改良MS+KT0.2 mg·L-1+6-BA0.3 mg·L-1+NAA0.1 mg·L-1,分化率为37.8%,牡丹愈伤组织不定芽在1/2MS+IBA0.2 mg·L-1条件下能够诱导生根,诱导率为最高的13.3%。本研究结果将为进一步开展江南牡丹的高效再生体系和转基因育种等研究提供技术支持和理论依据。

In order to study the effect of different medium and different plant hormone on callus induct and plant regeneration,in this study,young stems of the cultivar fengdan were used as explants to induce callus and its regeneration system was successfully established via callus pathway. The results showed that the best medium was modified MS,the induction rate of callus was 93. 3%,the optimum medium of callus induction were the modified MS ＋2,4-D0. 2 mg·L- 1＋ 6-BA2. 0 mg·L- 1＋ NAA0. 3 mg·L- 1. KT 0. 2 mg·L- 1and hydrolyzed Casein 300 mg·L- 1was an optimum concentration for callus differentiation. The best medium of callus differentiation were modified MS ＋KT0. 2 mg·L- 1＋ 6-BA0. 3 mg·L- 1＋ NAA0. 1 mg·L- 1,the differentiation rate was 37. 8%. The roots were induced after shoots were transferred to 1 /2 MS medium containing 0. 2 mg · L- 1IBA. The induction rate was 13. 3%. Our research results will provide theoretical basis and technology for further research on efficient regeneration system and transgenic breeding of peony.