森林草莓与栽培草莓体外抗氧化能力比较

Comparison on Antioxidant Capacity in vitro of Fragaria vesca and Strawberry Cultivars

为了鉴定草莓抗氧化物质含量和抗氧化能力,筛选具有较高营养价值的草莓遗传资源,以森林草莓和10个栽培草莓品种为试材,测定抗氧化物质,如总多酚、类黄酮、花青素、维生素C含量;采用2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS)清除法、铁离子还原能力(FRAP)测评了不同试材的体外抗氧化能力。结果表明,森林草莓抗氧化物质含量和抗氧化能力要远高于测试的栽培草莓;在10个栽培品种中,哈尼和凤冠的营养品质表现较好;总多酚含量与抗氧化能力呈显著正相关关系。主成分分析表明总多酚是草莓抗氧化能力的重要物质基础,即花青素与维生素C也是草莓抗氧化能力的主要组成参数。遗传背景对于草莓的抗氧化物质和抗氧化能力起重要作用;森林草莓的抗氧化能力高于测试的栽培品种,具有进一步开发和综合利用潜力。

The study aims to better characterize the antioxidant capacity of strawberry and screen the genetic source with high nutritional quality. The antioxidant content of Fragaria vesca and ten strawberry cultivars were determined and compared by measuring the total phenolics,total flavanoids,anthocyanins and vitamin C. The antioxidant capacity in vitro of strawberries were also tested and compared by using ABTS [2,2＇- Azino-bis（ 3- ethylbenzothiazoline- 6-sulfonic acid） diammonium salt] radical cation scavenging and ferric reducing antioxidant power（ FRAP） assays. The results indicated that F. vesca had higher antioxidant content and antioxidant capacity than the other cultivars. Based on the antioxidant content and antioxidant capacity,two culitvars ＂Honeoye＂and ＂Fengguan＂had the more satisfactory nutritional quality among ten cultivars. The total phenolics content and antioxidant capacity were significantly positive correlation. Principal component analysis（ PCA） indicated that total phenolics were the most important materials for antioxidant capacity of strawberry. Meanwhile,total anthocyanins and vitamin C were also the major contribution factors.This study showed that the genetic background played an important role in the antioxidant content and antioxidant capacity of strawberry fruits. F. vesca had higher antioxidant capacity than all cultivars in this study,which is the potential source for producing health-promoting products.