鄂西北及周边地区汉族人群D2S1338和D19S433基因座多态性分析与调查

Analysis of polymorphism of D2S1338 and D19S433 loci in Han population in northwest Hubei province

目的采集鄂西北及周边地区汉族人群无关个体血样,进行STR等位基因座D2S1338和D19S433多态性分析。方法应用chelex提取DNA,AmpFISTR Identifiler试剂盒扩增,毛细管电泳分型。结果在被检测的387位无关个体中等位基因座D2S1338和D19S433分别检出14和17个等位基因型以及两个等位基因的分布频率,基因座等位基因分布频率符合Hardy-Weinberg平衡。结论得到一些适合本地区应用的等位基因的频率,为以后司法物证鉴定工作提供参考。

Objective To analyze the distribution frequency of D2S1338 and D19S433 loci alleles in region of Han Nationality around Northwest Hubei Province based on the particular area population database. Methods DNA was extracted from sample using chelex-100, amplified by AmpFISTR Identifiler kit and typed by capillary electrophoresis. Results Among 387 unrelated individuals, 14 alleles were detected in D2S1338 locus and 17 alleles in D19S433 locus. The genotype frequencies of these alleles were consistent with Hardy-Weinberg equilibrium. Conclusion Allele frequencies acquired in this study nrovides a reference for evidence identification of forensic science in Shivan.