猪胆囊中沙门菌L型的分离培养与invA基因检测

Isolation and invA Gene Detection of Salmonella L-forms in pig gallbladder

为了解猪胆囊中沙门菌L型携带情况,在贵阳市屠宰场采集970例健康生猪的胆囊组织与胆汁标本,用常规细菌学方法和非高渗分离培养法分离沙门菌及其细菌L型,用PCR和核酸序列分析方法对稳定L型纯培养物进行沙门菌的invA基因检测。结果显示,970例生猪胆囊标本未检出沙门菌细菌型,细菌L型检出率为8.25%;80例细菌L型分离物中有50例invA检测阳性,占5.15%;占细菌L型阳性分离物62.50%。研究结果为生猪胆囊沙门菌L型感染的流行病学及其检查提供了依据。

To understand the situation of Salmonella L-forms within pig gallbladder,the samples of 970 gallbladder tissues and bile were collected from the healthy pigs at the slaughter house in Guiyang city,and Salmonella spp.and the bacterial L-forms were isolated from the gallbladders by the routine bacteriologi-cal method and the non-high osmotic isolation technique.The L-forms derived from Salmonella spp.in the stable L-form isolates were identified by PCR with the primers of Salmonella specific gene invA,and the positive products of PCR were analyzed by DNA sequencing.The results showed that no any bacterial form of Salmonella spp.was isolated from 970 samples of the pig gallbladder,but the positive rate of the bacteri-al L-form was for 8.25% in the specimens.The positive rate of gene assay for Salmonella invA was for 5.15% in the 970 specimens or for 62.50% in the stable L-form positive isolates.This research provide the basic epidemiological data and inspection of Salmonella L-form infections in pig gallbladder.