牛副流感病毒3型N基因的克隆与系统进化分析

Cloning and Phylogenetic Analysis of N Gene of Bovine Parainfluenza Virus Type 3

扩增大庆地区分离的牛副流感病毒3型的N基因,并进行同源性分析。设计特异性引物,通过聚合酶链反应扩增牛副流感3型N基因,将该基因分别连接到克隆载体的BamHⅠ、HindⅢ酶切位点中,测定插入片段的核苷酸序列,并利用分子生物学软件进行同源性分析。序列分析结果表明,扩增获得BPIV-3-N基因全长1 548bp,编码515个氨基酸,该N段基因核苷酸序列及所推导的氨基酸序列与GenBank中日本分离株910N基因序列同源性较高,核苷酸同源性为99.7%,氨基酸同源性为100%。

The N gene of bovine parainfluenza virus type 3 from Daqing was amplified and its homology was analyzed.A pair of primers were designed and the N gene of BPIV-3 was amplified by PCR.The gene was linked to the restriction enzyme cutting sites of BamH I and Hind Ⅲ of cloning vector,the nucleotide se-quence of the inserted fragment was determined and analyzed by molecular biological software.The PCR a-nalysis results showed that the BPIV-3-N was 1 548 bp,and encoded 515 amino acids.It indicated that the BPIV-3-N gene shared high homology with BPIV-3-N of 910N isolated from Japan in Genbank,the homol-ogy of nucleotides was 99.7%,the homology of amino acids was 100%.