摘要
新乡市某猪场发生疑似猪瘟病例,为了鉴别其为疫苗毒还是野毒,采用细胞免疫化学方法和RT-PCR,并对其NS5B基因进行序列测定,序列比对并构建进化树,结果表明,猪只为猪瘟野毒感染,且分离的猪瘟病毒与石门(Shimen)株在基因序列上未发生大的变异。
The suspicious cases of classical swine fever occurred in a pig farm of Xinxiang.The virus was i-dentified by cell immunochemistry method and RT-PCR.The results of the sequence alignment and phylo-genetic tree of NS5B gene showed that the pigs were infected with classical swine fever virus field strain, and the gene sequence variation of this isolate was not obvious.
出处
《动物医学进展》
北大核心
2015年第3期120-123,共4页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31201877)
