2012年-2013年我国集约化猪场猪伪狂犬病病毒感染情况的调查

Investigation on Infection of PRV in Intensive Hoggeries of China During 2012 to 2013

为研究全国范围内中大型猪场猪伪狂犬病病毒(PRV)感染情况,在2012年—2013年,应用PCR方法及gE-ELISA试剂盒对采集自17个省126个集团或猪场的164份病料、2 212份血清进行猪伪狂犬病(PR)病原及血清野毒gE抗体的检测。结果表明,病料样品中,PRV野毒阳性率为34.1%,PRV野毒阳性猪场占检测猪场总数的38.6%;血清样品中,PRV野毒抗体阳性猪场48个,猪场阳性率为38.1%,总样品阳性率为14.96%;2013年的感染率比2012年的高,且冬、夏两季PRV野毒阳性率较高;保育猪的PRV野毒阳性率最高,为22.34%,种母猪群PRV野毒阳性率为14.32%;华东地区PRV阳性比例最高,南方地区的PRV野毒阳性率高于北方地区。通过本次调查,说明PRV在我国的猪场依然普遍存在,对猪场进行PR的控制和净化是非常必要的。

In order to investigate the porcine pseudorabies virus （PRV）infection situation in intensive hoggeries in China,2 212 serum samples were collected from 126 farms in 17 provinces （cities）during 2012 to 2013 and tested for PRV gE antibodies by ELISA kit.And 164 tissue specimens from 57 hoggeries were examined by PCR meth-od.The results showed that,PRV positive rate was 34.1% in tissue specimens,the positive rate of infected farms was 38.6%.The positive rate of PRV gE antibody was 14.96%,the positive rate of infected farms was 38.1%. The PRV infection rate in 2013 was higher than that in 2012,especially in the winter and summer.The positive rate of nursery pigs was the highest,22.34%.The positive rate of PRV gE antibodies in sows was 14.32%.The posi-tive rate of PRV was the highest in Eastern China.The positive rate of PRV was higher in North China than that in South China.The results of this investigation showed that PRV still generally existed in China,the PRV control and purification is necessary for the hoggeries.