间充质干细胞对哮喘大鼠肺组织Notch信号通路的表达调控

Regulation of mesenchymal stem cells expressing Notch signaling pathway of asthma

目的:研究间充质干细胞对哮喘的调控修复,观察对哮喘大鼠肺组织Notch信号通路表达的影响。方法:30只大鼠随机分为正常对照组、哮喘模型组和MSC移植组3组。造模结束后,制作肺组织石蜡切片进行病理学检查,实时荧光定量PCR(RT-PCR)方法检测Notch2和Jagged1 mRNA在大鼠肺组织中的表达水平,Western检测肺组织Notch2、Jagged1蛋白的表达。结果:哮喘组大量炎性细胞浸润,管腔狭窄,与哮喘组相比,MSC移植组气道炎症和黏液分泌显著下调,管腔黏膜无增厚,上皮细胞脱落减少,正常组无炎性改变。MSC移植组和正常组Notch2、Jagged1 mRNA表达较哮喘组下调,Notch2、Jagged1蛋白表达减少(P<0.05)。结论:间充质干细胞能影响哮喘大鼠中Notch信号通路的表达,对哮喘的治疗发挥一定的作用。

Objective ：To study the regulation of MSCs on the asthma;and to observe the expression of Notch signal in lung tissue of asthmatic rats. Methods ： 30 rats were randomly divided into 3 group ： normal control group, asthma model group and MSC transplantation group. Making paraffin sections of lung tissue for pathological examination, quantitative real-time PCR （RT-PCR） were used to identify the expression of Notch2 and Jaggedl mRNA in the rat lung tissue, and the expression of Notch2, Jaggedl were measured by Western blot. Results： Compared with normal control group and MSC transplantation group, inflammatory cell infiltration and narrower airway were observed in asthma model group . The lever of Notch2 ,Jaggedl in asthma model group was higher than MSC transplantation group and normal control group（ P〈0.05 ）. Conclusion： Mesenchynml stem cells can affect the expression of the Notch signaling pathway in asthmatic rats, and play a role in the treatment of asthma.