HPLC、HPLC-MS在经方成分分析及Co-IP、激光共聚焦在经方药理机制研究中的应用

Introduction of Use of HPLC,HPLC-MS,Co-immunoprecipitation confocal laser technique in Classical Formula Research

经方用药精妙、临床疗效卓越,为历代医家所尊崇,也是科学研究者的重点研究对象。经方药物组成多样、成分复杂。经方的现代研究面临药理作用机制复杂、有效成分不明确等问题,从而制约了经方在现代医学领域的推广以及国际化的进程。HPLC及HPLC-MS联用技术广泛被应用到药物的分析、质控,同样适用于经方的成分分析。文章介绍了HPLC及HPLC-MS联用技术在经方成分及含量的鉴定中的应用,包括经方样品的前处理、经方成分的定量、定性分析以及经方的药物代谢研究等方面。免疫共沉淀技术可用于检测经方对蛋白之间的相互作用的影响,激光共聚焦技术可用于观察经方对蛋白、离子等的分布、转移的影响。结合免疫共沉淀与激光共聚焦技术,能更好地解析经方药理作用的分子机制,因此,文章对免疫共沉淀及激光共聚焦技术在经方现代药理研究中的应用也进行了介绍。

Classical formula, well-known for prominent clinical efficacy has complicated mechanism of action and unknown active substances due to varieties of compositions, which has constrained its in modern clinical medicine at a global platform.High per-formance liquid chromatography （ HPLC） and high liquid chromatography-mass spectrometry （ HPLC-MS） techniques, widely used in pharmaceutical analysis and quality control, has a multiple usage in active substance identification and content assessment of classical formula, ranging from sample pre-assessment handling, quantitative and qualitative analysis, pharmacokinetic research. Co-immunoprecipitation can be used to detect the effects of classical formula on the interactions between proteins, and confocal la-ser techniques can be used to observe the effects of classical formula on the distribution and transfer of proteins and ion.Therefore, the paper also briefed the use of those two techniques in the research of classical formula.