通痹外敷散对大鼠弗氏完全佐剂性膝关节炎的实验研究

Laboratory Research of Tongbi Application Powder for Treating Freund's Adjuvant Knee Arthritis in Rats

目的:观察中药通痹外敷散对弗氏完全佐剂性大鼠急性关节炎的影响,探讨其作用机制。方法:大鼠随机分为模型组及治疗组,分别于右膝关节腔内注弗氏完全佐剂0.1 m L,术前及术后1周、2周、3周分别测定白细胞介素-1β亚型(IL-1β)、透明质酸(HA)含量;测量右足趾关节以下部位的容积,计算各组治疗效果。结果:治疗2周后,治疗组IL-1β明显降低、HA含量明显升高,与治疗前及模型组比较有统计学意义(P<0.05)。治疗组足肿胀度及消肿时间明显优于模型组(P<0.05)。结论:通痹外敷散能有效治疗弗氏完全佐剂性大鼠急性关节炎,其作用可能是通过抑制IL-1β合成、增加HA含量而起到治疗作用。

Objective：To observe effect of Tongbi Application Powder on Freund’s adjuvant knee arthritis in rats and its mecha-nism.Methods：Rats were randomly divided into model group and treatment group, sc.injected Freund’s adjuvant 0.1ml into right knee, then measured interleukin-1 beta （IL-1 β）, hyaluronic acid （HA） before surgery,1wk, 2wk, 3wk after surgery;measured volume below the right toe.Results：After wk2 , treatment group had lower IL-1 beta and higher HA levels, significantly different compared with that before treatment and model group （ P〈0.05） .The treatment group’s extent of foot swelling and dura-tion was significantly better than that of model group, （ P〈0.05） .Conclusion：Tongbi Application Powder is effective for treating Freund’s adjuvant arthritis in rats, the effect may be induced by inhibiting IL-1 βsynthesis and increasing HA level.