高效液相色谱法测定银花感冒冲剂中绿原酸含量

Determination of the Contents of Chlorogenic Acid in Yinhuaganmao Granules by HPLC

目的：建立测试银花感冒冲剂中绿原酸含量的高效液相方法，并对此方法进行系统的方法学验证，以确保应用该方法测试的结果准确、可靠。方法：采用色谱柱：Agilent TC－C18柱（4．6 mm ×150 mm，5μm，Agilent，美国），流动相：甲醇－0．5％磷酸溶液（15∶85），检测波长为327 nm。结果：绿原酸在25～800μg／mL范围内线性关系良好，得到线性回归方程为Y＝27．88X＋2．128，相关系数为0．9998（n＝6）；低、中、高浓度精密度的RSD值分别为0．59％、0．58％、0．44％；重复性RSD为0．26％；低、中、高浓度的加样回收率分别为99．24％、99．43％、99．75％；供试品溶液室温放置8 h稳定， RSD为0．29％。结论：本方法测定银花感冒冲剂中绿原酸的含量准确、可靠，操作简单、用时短，可用于银花感冒冲剂中绿原酸的含量测定。

Objective：To establish a high performance liquid phase method to measure the content of chlorogenic acid of Flos Lonicerae in Ganmao granules,and to ensure that the results are accurate and reliable by systematic methodology.Methods：The chromatographic column：Agilent TC-C18 column （4.6 ×150 mm,5 μm,Agilent,American）,a mobile phase of methanol：-0. 5%phosphoric acid solution （15：85）,the detection wavelength was 327 nm.Results：The chlorogenic acid in 25ug mL-1 -800ug· mL^-1 linear relationship was good,the linear regression equation was Y =27.88X＋2.128,correlation coefficient was 0.999 8 （n =6）;low,high concentrations of RSD precision,values were 0.59%,0.58%,0.44%;repetitive RSD 0.26%;low,recover-ies.The high concentration were respectively 99.24%,99.43%,99.75%;the test solution was placed at room temperature for 8 h,and RSD was 0.29%.Conclusion：This method for determination of the content of chlorogenic acid in Flos Lonicerae cold gran-ule is accurate and reliable with simple operation and short time.It can be used for the determination of chlorogenic acid in Flos Lonicerae in Ganmao granules.