丹红、丹参和红花3种注射液抗氧化活性比较研究

Comparison of antioxidant activities of Danhong, Danshen and Honghua injection

[目的]对丹红、丹参、红花注射液的抗氧化活性进行比较,研究丹红、丹参、红花注射液抗氧化活性物质基础。[方法]采用清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基、铁离子还原、亚铁离子螯合实验测定3种注射液的抗氧化活性;用高效液相色谱法对丹参和丹红注射液中主要的抗氧化活性成分进行含量测定。[结果]丹红、丹参、红花注射液均具有DPPH自由基清除能力、铁离子还原能力、亚铁离子螯合能力。[结论]丹红、丹参、红花注射液均具有良好的抗氧化活性,其物质基础与其总酚酸和总黄酮的含量密切相关。

[Objective] To compare the antioxidant activity of Danhong, Danshen and Honghua injection and investigate the material basis for the antioxidant activity. [Methods] DPPH free radical scavenging, ferric reducing ability, ferrous ion chelating ability experiments were used for evaluation of the antioxidant components of Danhong, Danshen and Honghua injection. The main antioxidant components in Salvia and Danhong injection were determined by HPLC. [Results] Danhong, Danshen and Honghua injection had the DPPH free radical scavenging, ferric reducing ability, ferrous ion chelating ability. [Conclusion] The results suggest that Danhong, Danshen and Honghua injection have good antioxidant activity, which is closely related to the material basis of their total phenolic content of total flavonoids.