Smad7转染人骨髓间充质干细胞的初步实验研究

Primary research of human bone marrow mesenchymal stem cells transfected by Smad7

目的将Smad7转染到人骨髓间充质干细胞(BMMSC),并进行绿色荧光标记,将其植入兔青光眼手术模型,观察BMMSC存活情况。方法采用BP反应和LR反应,将绿色荧光标记的Smad7基因片段通过噬菌体插入BMMSC中,通过集落筛选,选出阳性表达细胞株。15只新西兰大耳白兔行小梁切除,局部予以BMMSC,观察细胞存活情况。结果BMMSC的Smad7表达稳定,绿色荧光标记成功。BMMSC在兔活体小梁存活,荧光表达满意,眼压稳定。结论绿色荧光标记的Smad7能稳定转染到BMMSC,在兔小梁切除术模型表达稳定。

Objective Human bone marrow mesenchymal stem cells were transfected by Smad7 and labeled with green fluorescent mark.BMMSCs were implanted into rabbit glaucoma operational model and observed surviving condition.Methods Through BP and LR reaction,Smad7 with green fluorescent mark was inserted into human bone marrow mesenchymal stem cells by bacteriophage,filtered positive colony and picked out cell line.15 New Zealand white rabbits were enforced trabeculectomy with BMMSC,then following up cell survival condition.Results Smad7 expressed stable in human bone marrow mesenchymal stem cells with satisfactory green fluorescent mark.BMMSC survived in rabbit trabecula with stable green fluorescent and effective ocular press.Conclusion Smad7 with green fluorescent mark could be inserted into human bone marrow mesenchymal stem cells stably,and has effective results in rabbit model.