HMGB1 RAGE蛋白在肝癌中的表达及临床意义

Expression and Clinical Significance of HMGB1 and RAGE in Hepatocellular Carcinoma

目的:研究原发性肝细胞癌中HMGB1及受体RAGE的表达与肝癌发生发展,侵袭转移的关系。方法:采用免疫组织化学及计算机图像分析技术方法检测肝癌旁及肝癌组织中HMGB1和RAGE的表达情况,及其与原发性肝细胞癌临床病理因素的关系。结果:HMGB1、RAGE在原发性肝细胞癌组织中存在协同高表达(r S=0.470,P=0.000),二者与肝癌患者性别、年龄、肿瘤大小无关(P>0.05)。肝癌伴淋巴结转移组HMGB1、RAGE的表达明显高于肝癌无淋巴结转移组,肝癌肿瘤组织包膜不完整者HMGB1、RAGE的表达高于包膜完整者,HMGB1表达随Edmondson分级的增加而增高,而RAGE与表达Edmondson分级无相关关系。结论:HMGB1和RAGE蛋白的表达与原发性肝细胞癌的临床病理学特征密切相关,HMGB1与其受体RAGE的结合可能参与了原发性肝细胞癌的侵袭和转移。HMGB1及其受体RAGE可能是促进原发性肝细胞癌发生、转移的重要信号通路。

Objective：To investigate the relation between the HMGB 1 （ high-mobility group box-B1） and RAGE （ receptor for advanced glycation end product ） in the development hepatocellular carcinoma （HCC）.Method：The expression and clinicopathological factor of HMGB 1 and RAGE protein were detected with immunohistochemistry and image analysis （ IA） techniques in 30 HCC speciments and tumor-adjacent-tissurs（ P〈0.05） .Result：The expressions of HMGB 1 and RAGE protein were coordinately increased （ rS =0.470,P=0.000）,expressions were not significantly related to the size of HCC and age ,gender and tumor size.The expression of HMGB1 and RAGE protein was higher in HCC with regional lymph nodes metastasis than primary HCC tissues with no regional lymph nodes metastasis （P〈0.05）.The expression of HMGB1 and RAGE protein was higher in HCC with complete envelope than primary HCC tissues with incomplete envelope （P〈0.05）.From the well differentiatiated HCC tissues to the poor differentiated tumor tissues , the expres-sions of HMGB1 and RAGE protein were increased （P〈0.05）.the expression of RAGE was not significantly related to the differentiatiated HCC tissues .Conclusion：HMGB1 protein and its receptor RAGE is highly co-expressed in HCC tissue , which may play an important role in the development and progress of HCC .The main signaling pathway is activated through the interaction of HMGB 1 with its receptor for RAGE in the de-velopment and progress of HCC .