维药野西瓜对佐剂性关节炎小鼠治疗效果及机制研究

Research on anti-inflammatory mechanism on the adjuvant arthritis mouse model by Uighur medicine Capparis Spinosa L

目的观察维药野西瓜治疗佐剂性关节炎(AA)小鼠的效果及机制。方法将40只雄性昆明鼠随机分为空白对照组、模型组、雷公藤组(雷公藤多甙片0.01 mg/kg)及野西瓜组。除空白对照组外,其余均采用完全弗氏佐剂制作AA小鼠模型,空白对照组及模型组灌胃生理盐水,雷公藤组和野西瓜组分别灌胃相应药物,连续灌胃25 d后,观察各组小鼠足肿胀、足踝关节组织病理变化及血清中白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)表达情况。结果药物干预25 d后,与模型组比较,雷公藤组及野西瓜组小鼠足趾肿胀程度均明显改善(P均<0.05),血清IL-6、TNF-α水平均明显降低(P均<0.05),组间比较差异无统计学意义。结论维药野西瓜对AA关节炎小鼠具有良好的抗炎作用,与其降低血清中IL-6及TNF-α的水平有关。

Objective It is to observe the anti-inflamatory effect of Uighur Medicine Capparis Spinosa L. on adjuvant ar- thritis（AA） in mice and to explore its mechanism. Methods 40 male Kunming mice were randomly divided into blank control group, model group, Tripterygium wilfordii group （ Tripterygium wilfordii polyglycoside 0. 01 mg/kg） and Capparis Spinosa L. group. The mice in every group except blank control group were made into AA models by complete Freund＇ s adjuvant. Blank control group and model group were given normal saline by intragastric administration, and Tripterygium wilfordii group and Capparis Spinosa L. group were given respective drugs by intragastric administration. The changes of ankle swelling, pa- thology of anklebone and the level of IL - 6 and TNF -α in serum in the mice were observed after 25 days＇ intragastric admin- istration. Results After 25 days＇intervention with the drugs, compared with model group, ankle swelling, pathology of ankle- bone of the mice in Tripterygium wilfordii group and Capparis Spinosa L. group were all improved （ P 〈 0.05 ） , there was no significant difference between the two groups. Conclusion Capparis Spinosa L. has good antiarthritic effects on AA in the mice, the mechanism may be associated with its significantly decreasing effect on the levels of IL - 6 and TNF -α in serum.