绵麦冬皂苷的提取分离及免疫活性研究

Extraction,Separation,and Immunoregulatory Activity of Saponins from Ophiopogon japonicus(L.f.) Ker-Gawl

采用正交试验优化超声波辅助提取绵麦冬总皂苷工艺,经溶液萃取得皂苷初步纯化物,用HZ-801树脂进一步分离得皂苷纯化物,并对皂苷纯化物进行初步的定性分析和色谱分析,最后对皂苷初步纯化物进行免疫活性研究。结果表明,超声波辅助提取100 g绵麦冬的最佳工艺为:提取温度60℃,乙醇体积分数70%,液料比15∶1(m L∶g),提取时间为2 h,提取次数为2次,总皂苷得率为(0.508±0.23)%。经树脂纯化可得3种不同的皂苷组分,都为甾体皂苷(麦冬皂苷B、A、C),得率分别为15.4%、40.6%和12.6%,纯度分别为72.7%、86.4%和83.3%。皂苷初步纯化物具有显著的巨噬细胞活化能力,促进一氧化氮和白细胞介素生成等免疫活性。

The extraction process of the total saponins in the root of Ophiopogon japonicus by ultrasonic wave was optimized through orthogonal test. Then the saponins were separated by the HZ-801 absorbent resin and the purified samples were identified with chemical and chromatographic method. At last,the immunoregulatory activities of total saponins were studied in vitro. The optimal combination parameters of the extracting technology for 100 g tuber of Ophiopogon japonicus were extracting temperature60 ℃,ethanol volume fraction 70%,the ratio of ethanol to material 15 ∶ 1,extracting time 2 h,and extracting twice. The yield of total saponins was（ 0. 508 ± 0. 23） %. It was found that three different fractions,named ophiopogonin B（ 15. 4%）,A（ 40. 6%）,and C（ 12. 6%）,were proved to be steroidal saponins by chemical test. Saponin,which was purified preliminarily by ether and n-butyl alcohol and sodium hydroxide,had prominent immunoregulatory activities,i. e.,remarkable macrophageactivating capability and nitric oxide and interleukin-1 producing ability.