宫颈鳞癌患者血清miRNA-574-5p的水平及其诊断价值

Serum miRNA-574-5p level and its diagnostic value in patients with cervical squamous cell carcinoma

目的研究宫颈鳞癌患者血清miRNA-574-5p的表达情况及其在宫颈鳞癌诊断中的应用。方法采集80例初治宫颈鳞癌患者及20例体检健康妇女的血清标本,提取RNA后采用实时荧光定量PCR检测血清miRNA-574-5p水平,并绘制ROC曲线图;采用Spearman秩相关分析miRNA-574-5p水平与宫颈鳞癌临床病理特征的关系。结果宫颈鳞癌患者血清中miRNA-574-5p相对表达水平高于正常对照组(P<0.05);ROC曲线下面积0.801,最大约登指数为0.49,灵敏度74%,特异度75%,对应诊断界值为2.83。宫颈癌患者血清miRNA-574-5p相对表达水平与宫颈癌的临床分期、病理分级及肿瘤大小呈正相关(P<0.05),而与年龄无显著相关性(P>0.05)。结论宫颈癌患者miRNA-574-5p相对表达水平增高,为宫颈癌的临床诊断提供了重要参考,具有重要的临床应用价值。

Objective To investigate the expression level of serum miRNA-574-5p in the diagnosis of cervical squamous cell carcinoma. Methods The serum samples were collected from 80 newly-treated patients with cervical squamous cell carcinoma and 20 healthy women （controls） to obtain RNA for detecting serum miRNA-574-Sp level by real-time RT-PCR,and its diagnostic value was analyzed by the receiver operating characteristic（ ROC ） curve. The relationship between serum miRNA-574-5p level and clinicopathologic features of cervical squamoas cell carcinona was analyzed by Spearman rank correlation. Results The relative expression level of serum miRNA-574-Sp was significantly higher in patients with cervical squamous cell carcinoma than that in normal controls（P 〈0.05）. The area under ROC curve was 0.801,and the nmx Youden index was 0. 49. The sensitivity and specificity of serum miRNA-574-Sp were 74% and 75% ,respectively,and the corresponding cut-off value was 2.83. The relative expression level of serum miRNA-574-Sp had a significantly positive correlation with the International Federation of Gynecology and Obstetrics（FIGO） stage and pathological grade, and tumor size （P 〈 0.05 ） amomg patients with cervical carcinoma, but had no significant correlation with age （ P 〉 0.05 ）. Conclusion The relative expression level of miRNA-574-Sp increases in patients with cervical cancer, which provides important references for the diagnosis of cervical cancer. And it has important clinical value.