尘肺以及尘肺合并结核病肿瘤坏死因子α-238基因多态性研究

Research on gene polymorphism of tumor necrosis factor-α-238 in patients with pneumoconiosis,pneumoconiosis combined with tuberculosis

目的探讨尘肺及尘肺合并结核病患者肿瘤坏死因子-α(TNF-α)-238基因多态性。方法以103例尘肺患者、51例尘肺合并结核病患者以及60例粉尘接触者为研究对象,应用聚合酶链反应-限制性片断长度多态性技术检测TNF-α-238位点多态性。结果 3组TNF-α-238位点基因型分布、等位基因频率比较,差异均无统计学意义(P>0.05)。结论 TNF-α-238基因多态性可能与尘肺、尘肺合并结核病发病无关联。

Objective To investigate the gene polymorphism of tumor necrosis factor-α（TNF-α）-238 in patients with pneumoconiosis,pneumoconiosis combined with tuberculosis. Methods One hundred and three pneumoconiosis patients ,51 pneumoconiosis combined with tuberculosispatients and 60 dust-exposed workers were enrolled in the study. Polymerase chain reacfion-reslriction fragment length polymorphism（PCR-RFLP） technique was used to detect the polymorphism of TNF-α-238. Results There was no significant difference in genotype distribution and allele frequency of TNF-α-238 among three groups（ P 〉 0.05 ）. Conclusion TNF-α-238 gene polymorphism might not correlate with pneumoniosis, pneumoconiosis combined with tuberculosis.