摘要
利用RT-PCR技术从草鱼性腺中克隆出两种促性腺激素受体基因(FSHR和LHR),采用N-J法构建了系统进化树。通过RT-PCR及Real-time PCR技术对FSHR和LHR在成体草鱼不同组织中的时空表达进行分析,发现FSHR和LHR在草鱼卵巢、精巢中表达明显,表达量随着卵母细胞的发育逐渐增高,以上结果为进一步探讨GTHR在鱼类生殖周期中的生理功能奠定了基础。
Two types of gonadotropin receptors(FSHR and LHR) were cloned from the gonads of grass carp by RT-PCR. The phylogenetic tree of GTHR between different species by N-J method was constructed. The temporal and spatial expression of different tissues in adult grass carp by RT-PCR and Real-time PCR was analyzed. The result indicated that FSHR and LHR were much higher expressed in gonads, and the expression level was significantly increased in mature oocytes. These results laid a foundation for the further study on GTHR reproductive functions in fish.
出处
《生命科学研究》
CAS
CSCD
2015年第1期39-43,共5页
Life Science Research
基金
国家自然科学基金项目(31272261
31072199)
留学回国人员科研启动基金资助项目(教外司留(2011)1568号)
关键词
草鱼
促性腺激素受体
基因克隆
表达分析
grass carp
gonadotropin receptors
gene cloning
expression analysis