草鱼促性腺激素受体的克隆及表达分析

Molecular Gene Cloning and Expression of Gonadotropin Receptors from Ctenopharyngodon idella

利用RT-PCR技术从草鱼性腺中克隆出两种促性腺激素受体基因(FSHR和LHR),采用N-J法构建了系统进化树。通过RT-PCR及Real-time PCR技术对FSHR和LHR在成体草鱼不同组织中的时空表达进行分析,发现FSHR和LHR在草鱼卵巢、精巢中表达明显,表达量随着卵母细胞的发育逐渐增高,以上结果为进一步探讨GTHR在鱼类生殖周期中的生理功能奠定了基础。

Two types of gonadotropin receptors（FSHR and LHR） were cloned from the gonads of grass carp by RT-PCR. The phylogenetic tree of GTHR between different species by N-J method was constructed. The temporal and spatial expression of different tissues in adult grass carp by RT-PCR and Real-time PCR was analyzed. The result indicated that FSHR and LHR were much higher expressed in gonads, and the expression level was significantly increased in mature oocytes. These results laid a foundation for the further study on GTHR reproductive functions in fish.