摘要
＂超级细菌＂耐甲氧西林金黄色葡萄球菌（methicillin resistant Staphylococcus aureus,MRSA）是诱发连续腹膜透析患者腹膜炎的常见细菌,且治疗困难。目前缺少MRSA腹膜炎动物模型。腹腔注射2×10^9-2×10^10CFU/mL 7组不同浓度的MRSA感染小鼠,观察小鼠死亡时间,测定肝脏与脾脏细菌定植量,进行肝、脾病理分析,确定适宜的建模浓度。研究发现,小鼠感染细菌浓度最小致死剂量为每只2×10^9CFU,最适建模浓度为每只1.4×10^9CFU。结果表明建立了耐甲氧西林金黄色葡萄球菌小鼠腹膜炎模型,为MRSA致腹膜炎的致病机制研究、疫苗的研制提供实验基础。
＂Super bacteria＂ methicillin resistant Staphylococcus aureus（MRSA） is a frequent cause of peritonitis in continuous peritoneal dialysis,and the treatment is difficult. Meanwhile, MRSA peritonitis animal models are lacking. It was performed with mice divided into seven groups and given intraperitoneal injection2 ×10^9-2 ×10^10CFU/m L of MRSA. At the same time, the time of death was recorded. Besides, the bacteria amount of liver and spleen was measured and the pathological analysis of liver and spleen was performed.Then appropriate modeling concentration was determined. The results showed that the minimal lethal dose of the clinical isolated MRSA was 2×10^9CFU each mouse and the optimal concentration of modeling was 1.4×10^9CFU each mouse. The mouse peritonitis model of methicillin-resistant staphylococcus aureus was constructed successfully, which provided a reliable animal model for the research of peritonitis caused by MRSA and development of the vaccine.
出处
《生命科学研究》
CAS
CSCD
2015年第1期54-58,共5页
Life Science Research
基金
广东省自然科学基金项目(S2013010014393)
