摘要
目的探讨全血标本冷藏保存1周对血清乙肝病毒DNA（HBV DNA）复检结果的影响。方法采集乙肝患者外周全血150例,及时2~8℃保存,分别于采血后第1天和第7天用实时荧光定量PCR法（FQ-PCR）检测血清HBV DNA水平。结果150例全血标本第1天、第7天时血清HBV DNA载量分别为：5.50±1.46、5.64±1.53,结果呈小幅升高趋势,但两组差异无统计学意义（P〉0.05）;以第1天结果为基准,第7天时结果偏倚绝对值为（4.26±0.03）%,其中15例偏倚大于±7.5%。结论以分析中质量控制为前提,全血标本冷藏保存1周对血清HBV DNA复测结果的影响符合ISO 15189室内质控要求,能满足临床追加检测或复查申请;操作误差和FQ-PCR方法学的不足仍是造成复测较大偏差的最主要原因。
Objective To explore the influence of the whole blood samples cold storage time on detecting serum hepatitis B virus DNA（ HBV DNA） by real-time fluorescent quantitative PCR（ FQ-PCR）. Methods Whole blood samples of 150 patients with hepatitis B virus were collected in vacuum tubes containing coagulant. These samples were centrifuged in 1 hour and stored in cold storage（ 2-8℃）. The serum HBV DNA levels in the samples were detected by FQ-PCR at 1stday and at 7thday. Results The HBV DNA load levels of 150 patients were 5. 50 ± 1. 46 and 5. 64 ± 1. 53 at 1stand 7thday respectively. These results showed an upward trend,but there was no significantly difference between two groups（ P 〉 0. 05）. Compared with the results of the first day,it had a quiet tolerance of（ 4.26 ±0.03） % at the 7thday,and among these 150 samples,more than 7. 5% deviation was found in 15 samples.Conclusion On the premise of the quality control in the course of FQ-PCR,the influence of the whole blood samples’ cold storage time on detecting serum HBV DNA fits the requirements of ISO1 5189,which can meet the clinical needs. Inexact operation and methodological weaknesses are the main causes of deviations.
出处
《标记免疫分析与临床》
CAS
2015年第2期152-154,共3页
Labeled Immunoassays and Clinical Medicine
