温州羊栖菜(Hizikia fusiformis)野生与选育种群ISSR遗传研究

ISSR analysis of genetic variation to Sargassum fusiformis wild and breeding strains in Wenzhou

采用ISSR技术对温州2个羊栖菜野生种群和3个选育养殖品系进行了遗传多样性及遗传变异分析并构建了聚类分析图。结果显示:6条ISSR引物共获得76个位点,56个多态性位点,多态性位点百分率为74.67%;Nei's基因多样性为0.1823,Shannon's指数为0.2712,均高于个体水平;种群内和种群间Nei's基因多样性计算遗传分化水平(Gst)为0.5529;种群间的基因交流为Nm=0.3341;Shannon's信息指数显示野生种群的遗传变异性及遗传多样性普遍高于经过选育的养殖种群;同时说明经过选育,每个养殖品系的性状趋于稳定;聚类分析图显示3个选育养殖品系与2个野生种群分开,表明经过选育工作,野生羊栖菜种群与养殖羊栖菜种群呈现出明显差异。

The inter-simple sequence repeat （ ISSR） molecular marker technique was used to investigate genetic di-versity, genetic variation to 2 wild stocks and 3 breeding strains in Hizikia fusiformis of south Zhejiang.The results showed that：a total of 76 loci and 56 polymorphic loci were obtained from 6 primers which had been screened and P%（the percentage of polymorphic loci） was 74.67%.Nei＇s gene diversity was 0.1823.Shannon＇s information in-dex was 0.2712.The values were higher than the individual level.Population within and between populations Nei＇s genetic diversity calculation level of genetic differentiation （ Gst） was 0.5529, the genetic differentiation between populations was greater than the population differentiation.Gene exchange between the populations （ Nm ） was 0.3341;Shannon＇s information index showed that the genetic variability and genetic diversity of wild populations were generally higher than breeding populations.Results also showed that after breeding, the traits of each breeding strains tends to be stable.Clustering figure showed that three breeding strains were separated from two wild popula-tions and they presented significant difference after breeding.