光果西南杨带芽茎段的组培技术研究

The Tissue Culture Technique of Stems with Buds of Populus schneideri var. tibetica

以光果西南杨(Populus schneideri var.tibetica)带芽茎段为外植体,进行消毒灭菌处理、不定芽诱导、再生芽增殖、再生苗壮苗以及生根后移栽的一系列试验,对组培过程中最佳培养方法进行研究,筛选出了适合植株分化、生根的培养基成分。结果表明:酒精消毒45 s,升汞消毒8 min的灭菌效果最好;在MS+NAA 0.05+6-BA 2.0的培养基中芽诱导率达79%,10 d后腋芽萌发;在MS+NAA 0.1+6-BA 2.0的培养基中增殖效果最好,增殖系数达到5.8;经过培养基为MS+NAA 0.1+6-BA 0.5的壮苗过程之后,在1/2 MS+NAA 0.5的培养基中增根效果最佳。这将为光果西南杨苗木的大量繁殖以及遗传改良提供技术储备。

In this study,Populus schneideri var. tibetica＇s stems with buds were selected as explants. A se- ries of tests were conducted on disinfection and sterilization, the induction of adventitious buds, the prolif- eration of regeneration buds, strong regenerated seedlings, rooting and transplantation in order to study the best training methods during the tissue culture process, and to screen out medium components for plant differentiation and rooting. The results showed that 45 seconds of alcohol disinfection and 8 minutes of mercuric chloride sterilization worked best. The bud induction rate of explants reached 79% in MS ＋ NAA 0. 05 ＋ 6 - BA 2.0 medium, and the axillary bud sprouted after 10 days. The proliferation coefficient was up to 5.8 in MS ＋ NAA 0. 1 ＋ 6 - BA 2. 0 medium, displaying the best effects. The effect of promoting rooting was significant in 1/2 MS ＋ NAA 0. 5 medium after strengthening the regenerated seedlings in MS ＋ NAA 0. 1 ＋ 6 - BA 0.5 medium. This would provide technical reserve for the cultivation speed acceler- ation and genetic improvement of Populus schrteideri var. tibetica.