高效液相色谱法测定粮食中脱氧雪腐镰刀菌烯醇含量的不确定度分析

Uncertainty analysis of deoxynivalenol content in grain determined by HPLC

根据JJF 1059—1999《测量不确定度评定和表示》提供的不确定度分析思路,对高效液相色谱法测定粮食中脱氧雪腐镰刀菌烯醇的含量进行不确定度分析,为建立有效的质量控制方法提供参考。结果表明:本试验的扩展不确定度为0.063 mg/kg,从不确定度的各个分量比较,影响高效液相色谱测定粮食中脱氧雪腐镰刀菌烯醇含量的不确定主要来自其测量的重复性。

Based on JJF1059--1999 ＂ Evaluation of the Uncertainty of Determination and Expression＂, the uncertainty analysis was established for determining of deoxynivalenol （DON） content in grain by HPLC, offering references for building effective quality control methods. Results show that the uncertainty of this experiment is 0. 063 mg/kg. Comparison of each uncertainty analysis, the uncertainty of deoxynivalenol （DON） content in grain determined by HPLC mainly comes from the measurement repeatability.