STMN1在食管鳞状细胞癌侵袭和转移中的作用及其预后意义

Role of STMN1 in esophageal squamous cell carcinoma invasion and metastasis and its prognostic significance in human patients

目的研究STMN1蛋白在食管鳞状细胞癌组织中的表达及其在食管鳞状细胞癌侵袭和转移中的作用。方法应用免疫组织化学法检测150例食管鳞状细胞癌及癌旁组织中STMN1的表达水平,分析其与临床病理特征的关系。将STMN1siRNA通过瞬时转染于食管鳞状细胞癌TE-1细胞,使用qPCR和Western blot实验检测转染效果,通过划痕实验、Millicell小室侵袭和转移实验观察转染STMN1siRNA对TE-1细胞侵袭和转移能力的影响。结果 STMN1蛋白在食管鳞状细胞癌组织中高表达,且与TNM分期和淋巴结转移之间显著相关,STMN1表达阳性者预后显著差于表达阴性者。siRNA干扰STMN1的表达可明显抑制TE-1细胞的侵袭和转移能力。结论 STMN1促进了食管鳞状细胞癌侵袭和转移的发生并且对食管鳞状细胞癌患者的预后判断具有重要意义。

Objective To investigate the role of STMN1 in esophageal squamous cell carcinoma（ESCC） invasion and metas- tasis in human patients. Methods We quantified STMN1 in 150 paraffin-embedded ESCC primary tumor and paired adjacent noncancerous samples by immunohistochemistry. The association between STMN1 protein expression and the clinicopathological char- acteristics of ESCC was explored. The STMN1 siRNA（siSTMN1） or scramble siRNA（SCR） were transient transfected into ESCC TE-1 cells. The mRNA and protein levels of STMN1 were detected by qPCR and Western blot in the TE-1 cells of different groups. Wound-Healing Assay,in vitro invasion and metastasis assays were used to investigate the effect of silencing STMN1 on the invasion and metastasis of ESCC cells. Results Positive immunoreactivity for STMN1 was observed primarily in the cytoplasm of ESCC cells. The segregation of the ESCC patients into STMNl-positive and STMNl-negative groups significantly correlated with TNM stage and lymph node metastasis. Furthermore, we found that the patients with STMNl-positive ESCC had significantly worse prognoses than those with STMNl-negative ESCC. Down-regulation of STMN1 significantly reduced TE-1 cells invasion and metastasis. Conclusion We report that the dysregulation of STMN1 may play a key role in the regulation of invasion and metastasis in ESCC cells and that STMN1 is a potential biomarker for ESCC prognosis.