摘要在20世纪90年代初,美国的Tuerk等在实验室中发明了一种叫做指数富集配基的系统进化（systematic evolution of ligands by exponential enrichment,SELEX）技术,由此技术筛选出来的寡核苷酸命名为适配子（aptamers）。适配子可以和抗体媲美,甚至优于抗体。
1Tuerk C, Gold L. Systematic evolution of ligands by expo- nential enrichment: RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990,249(4968) : 505-510.
2Ellington AD,Szostak JW. In vitro selection of RNA mol- ecules that bind specific ligands [J]. Nature, 1990, 346 (6287) :818-822.
3Sefah K, Shangguan D, Xiong X, et al. Development of DNA aptamers using Cell-SELEX[J]. Nat Protoc, 2010,5 (6) : 1169-1185.
5Lee YJ, Lee SW. Regression of hepatocarcinoma cells u- sing RNA aptamer specific to alpha-fetoprotein[J].Bio- chem Biophys Res Commun,2012,417(1) :521-527.
6Syed MA, Pervaiz S. Advances in aptamers[J]. Oligonu- eleotides,2010,20(5) :215-224.
7Ni X, Castanares M, Mukherjee A, et al. Nucleic acid aptamers: clinical applications and promising new hori- zons[J]. Curr Med Chem, 2011,18(27) : 4206-4214.
8Handy SM,Yakes BJ,DeGrasse JA,et al. First report of the use of a saxitoxin-protein conjugate to develop a DNA aptamer to a small molecule toxin[J]. J Int Soc Toxinol, 2013,61 : 30-37.
9Hamula CL, Zhang H, Guan LL, et al. Selection of aptam- ers against live bacterial cells[J].Anal Chem, 2008,80 (20) :7812-7819.
10Wang P, Hatcher KL, Bartz JC, et al. Selection and char- acterization of DNA aptamers against PrP (Sc) [J]. Exp Biol Med(Maywood) ,2011,236(4) :466-476.
