白细胞介素17在肾移植排斥反应中的表达及意义

Expression and significance of interleukin-17 in rejection after kidney transplantation

背景:白细胞介素17是一种强效的炎性细胞因子,在急性排斥反应研究中较多,而在慢性排斥反应以及急性和慢性共同研究甚少。目的:通过研究白细胞介素17表达与移植肾发生排斥反应的相关性,探索白细胞介素17表达与排斥反应的关系。方法:选取解放军第309医院肾移植后穿刺及复查患者47例,根据患者病情分为肾功能稳定组(n=20)、急性排斥组(n=15)和慢性排斥组(n=12),另取健康体检人员15人作为正常组。应用实时荧光定量PCR检测4组受试对象外周血单个核细胞中白细胞介素17 mR NA的相对表达水平,分析其与血清肌酐和排斥时间的相关性。结果与结论:与正常组相比,急性排斥组与稳定组患者白细胞介素17 m RNA的表达水平差异有显著性意义;而慢性排斥组患者白细胞介素17 mR NA较稳定组升高了2.89倍(P<0.01)。急性排斥组及慢性排斥组患者白细胞介素17 mR NA表达水平与肌酐、移植后排斥时间均无明显相关性。提示白细胞介素17在排斥反应中存在显著性意义。

BACKGROUND： Interleukin-17 is a potent inflammatory cytokine, which has been greatly reported in acute rejection research but little in chronic rejection or acute and chronic rejection research. OBJECTIVE： By studying the correlation between interleukin-17 and renal allograft rejection, to explore the asociation between interleukin-17 and rejections. METHODS： in accordance with the inclusion criteria strictly, health physical examination persons （15 caess） and kidney transplantation recipients （47 caess） were choosed and divided into four groups： normal group （15 cases）, stable renal function group （20 cases）, acute rejection group （15 Cases）, and chronic rejection group （12 cases）. The relative expression levels of interleukin-17 mRNA in peripheral blood mononuclear cells of these four groups were detected by real-time fluorescence quantitative PCR. Then differences between four groups were compared and the correlations with the serum creatinine and the rejection time were analyzed. RESULTS AND CONCLUSION： The expression levels of interleukin-17 mRNA in the acute rejection and stable renal function groups were signfiicantly different from that in the normal group. The expression level of interleukin-17 mRNA in the chronic rejection group was increased by 2.89 times, showing a significant difference from the stable group （P 〈 0.01 ）. In the acute and chronic rejection groups, the expression level of interleukin-17 mRNA had no significant correlation with serum creatinine level and postoperative rejection time. These findings indicate that intedeukin-17 is of significance in renal allograft rejection.