摘要
背景:白细胞介素17是一种强效的炎性细胞因子,在急性排斥反应研究中较多,而在慢性排斥反应以及急性和慢性共同研究甚少。目的:通过研究白细胞介素17表达与移植肾发生排斥反应的相关性,探索白细胞介素17表达与排斥反应的关系。方法:选取解放军第309医院肾移植后穿刺及复查患者47例,根据患者病情分为肾功能稳定组(n=20)、急性排斥组(n=15)和慢性排斥组(n=12),另取健康体检人员15人作为正常组。应用实时荧光定量PCR检测4组受试对象外周血单个核细胞中白细胞介素17 mR NA的相对表达水平,分析其与血清肌酐和排斥时间的相关性。结果与结论:与正常组相比,急性排斥组与稳定组患者白细胞介素17 m RNA的表达水平差异有显著性意义;而慢性排斥组患者白细胞介素17 mR NA较稳定组升高了2.89倍(P<0.01)。急性排斥组及慢性排斥组患者白细胞介素17 mR NA表达水平与肌酐、移植后排斥时间均无明显相关性。提示白细胞介素17在排斥反应中存在显著性意义。
BACKGROUND: Interleukin-17 is a potent inflammatory cytokine, which has been greatly reported in acute rejection research but little in chronic rejection or acute and chronic rejection research. OBJECTIVE: By studying the correlation between interleukin-17 and renal allograft rejection, to explore the asociation between interleukin-17 and rejections. METHODS: in accordance with the inclusion criteria strictly, health physical examination persons (15 caess) and kidney transplantation recipients (47 caess) were choosed and divided into four groups: normal group (15 cases), stable renal function group (20 cases), acute rejection group (15 Cases), and chronic rejection group (12 cases). The relative expression levels of interleukin-17 mRNA in peripheral blood mononuclear cells of these four groups were detected by real-time fluorescence quantitative PCR. Then differences between four groups were compared and the correlations with the serum creatinine and the rejection time were analyzed. RESULTS AND CONCLUSION: The expression levels of interleukin-17 mRNA in the acute rejection and stable renal function groups were signfiicantly different from that in the normal group. The expression level of interleukin-17 mRNA in the chronic rejection group was increased by 2.89 times, showing a significant difference from the stable group (P 〈 0.01 ). In the acute and chronic rejection groups, the expression level of interleukin-17 mRNA had no significant correlation with serum creatinine level and postoperative rejection time. These findings indicate that intedeukin-17 is of significance in renal allograft rejection.
出处
《中国组织工程研究》
CAS
CSCD
2014年第51期8275-8280,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(81171753)~~