衰老大鼠持续游泳运动后学习记忆能力和IL-6、IL-10表达变化

Effects of Prolonged Swimming Exercise on the Learning-memory and Expressions of IL-6 and IL-10 in D-galactose-induced Aging Rats

目的:探讨持续游泳运动对D-半乳糖衰老大鼠学习、记忆以及IL-6、IL-10表达的影响。方法:采用D-半乳糖衰老模型,将雄性SD大鼠分为3组:(1)生理盐水对照组(n=10),腹腔注射生理盐水;(2)D-半乳糖安静组(n=10),腹腔注射D-半乳糖(100 mg/kg/d),连续8周;(3)D-半乳糖运动组(n=10),腹腔注射D-半乳糖,并进行持续游泳训练12周。采用Morris水迷宫实验评价大鼠学习记忆能力;免疫荧光染色和蛋白免疫印迹(Western-blotting)检测下丘脑白细胞介素6(IL-6)和白细胞介素10(IL-10)表达。结果:(1)Morris水迷宫实验结果显示,D-半乳糖安静组大鼠寻找平台潜伏期的时间较生理盐水对照组明显延长,在目标象限游泳时间百分比(29.42±2.12)显著低于生理盐水对照组(45.12±1.26)(P<0.05);D-半乳糖运动组潜伏期时间显著短于D-半乳糖安静组(P<0.05),目标象限游泳时间所占百分比(35.35±1.54)较D-半乳糖安静组增加。(2)IL-6免疫荧光染色和蛋白免疫印迹检测结果表明,D-半乳糖安静组IL-6表达较生理盐水对照组显著增加(P<0.05);D-半乳糖运动组IL-6阳性细胞数(8.32±0.43)较D-半乳糖安静组(11.54±0.52)显著减少(P<0.05),表明持续游泳运动有助于降低衰老模型大鼠促炎因子表达水平,抑制炎症反应。(3)IL-10免疫荧光染色和蛋白免疫印迹检测结果表明,D-半乳糖运动组IL-10阳性细胞数(11.22±0.53)较D-半乳糖安静组显著增加(P<0.05),表明持续游泳运动可上调抗炎因子表达水平。结论:持续游泳运动有助于改善衰老大鼠学习记忆能力,并可能通过抑制下丘脑炎症因子IL-6的表达,并提高抗炎因子IL-10的含量,抑制炎症反应,从而延缓衰老。

Objective The purpose of this study was to investigate the effect of exercise on the learning-memory capacity and the expression of IL-6 and IL-10 in D-galactose-induced aging rats. Methods Male Sprague-Dawley rats were divided into three groups：intraperitoneal injection of physiological saline sedentary group （C,n =10）,intraperitoneal injection of D-galactos sedentary group （A,n = 10）,and intraperitoneal injection of D-galactos swimming group （AS,n =10）. Rats in group AS were subjected to swimming for 12 weeks. The learning-memory of rats was evaluated by Morris water maze by the end of the experiment. The expressions of IL-6 and IL-10 in hypothalamus were examined by immunofluorescent staining and western-blotting. Results （I）The searching escape latency was significantly longer,and the percentage of time in target quadrant （29.42±2.12）significantly lower in group A than in group C （45.12±1.26）（P 〈 0.05）.（2）The number of IL-6 positive cells and protein level in group AS increased significantly as compared to the group C （P 〈 0.05）. （3）The number of IL- 10 positive cells and protein expression in group AS increased significantly as compared to the group A （P 〈 0.05）. Conclusion Prolonged swimming exercise elevates the learning-memory and delays the senescence of aging rats.