自发性高血压大鼠左心室肥厚心肌微小RNA-1及微小RNA-133的差异表达

The differences in expressions of miRNA-1 and miRNA-133 in the tissue of hypertrophic left ventricle of spontaneously hypertensive rats

目的观察自发性高血压大鼠(SHR)肥厚的左心室心肌组织微小RNA-1(miR-1)、微小RNA-133(miR-133)表达的变化。方法选取血压升高合并左心室肥厚(LVH)的17周龄雄性SHR为LVH组(n=10),以出现血压升高无LVH的8周龄雄性SHR作为高血压组(n=10),17周龄雄性WKY大鼠作为对照组(n=10)。通过HE染色,观察大鼠左心室心肌组织病理学改变,提取大鼠左心室心肌组织总RNA,实时荧光定量聚合酶链反应(qRT-PCR)检测miR-1和miR-133在SHR肥厚左心室心肌中的表达。结果与对照组比较,LVH组和高血压组的收缩压、舒张压升高[收缩压(188.5±13.6),(152.3±10.8)比(130.6±10.1)mm Hg,P<0.01;舒张压(123.5±15.3),(94.3±10.4)比(74.3±17.4)mm Hg,P<0.05]。对照组和高血压组左心室质量(LVM)/体质量及心肌细胞横径差异无统计学意义(均P>0.05);与高血压组比较,LVH组左心室心肌细胞肥大,心肌间质增多,LVM、LVM/体质量及左心室心肌细胞横径增大[分别为(956±33)比(684±24)mg,(2.87±0.15)比(2.17±0.14)mg/g,(15.32±0.61)比(12.01±0.43)μm,均P<0.05],左心室心肌组织miR-1表达升高,miR-133表达降低。结论SHR肥厚左心室心肌中的miR-1和miR-133在不同时期出现差异表达,表现为miR-1表达升高,miR-133表达降低。

Objective To investigate the differences in the expressions of miRNA-1（miR-1）and miRNA-133（miR-133）in the tissue of hypertrophic left ventricle of spontaneously hypertensive rats（SHR）. Methods17-week-old male SHR with left ventricle hypertrophy（LVH group,n=10）,8-week-old male SHR without LVH（SHR group,n=10）and 17-week-old male Wistar-Kyoto（WKY）rats（WKY group,n=10）were used in this study. HE staining was used to measure the pathological changes and quantitative real-time PCR（qRT-PCR）used to determine the expressions of miR-1and miR-133 in the left ventricular tissue. Results Compared with WKY group,systolic blood pressure（SBP）and diastolic blood pressure（DBP）were higher in LVH group and SHR group[（188.5±13.6）,（152.3±10.8）vs（130.6±10.1）mm Hg,P0.01;（123.5±15.3）,（94.3±10.4）vs（74.3±17.4）mm Hg;P0.05]. There were no statistical differences in left ventricular mass（LVM）/body mass and the transverse diameter of myocardial cells between WKY group and SHR group（all P0.05）. Compared with SHR group,left ventricular myocardial cells became marked hypertrophy,myocardial interstitium increased,LVM,LVM/body mass and transverse diameter of myocardium were larger in LVH group[（956±33）vs（684±24）mg,（2.87±0.15）vs（2.17±0.14）mg/g,（15.32±0.61）vs（12.01±0.43）μm,all P0.05]. In addition,the level of miR-1 significantly increased and the level of miR-133 significantly decreased in LVH group.Conclusion Different expressions of miR-1and miR-133 were detected in the left hypertrophic ventricular tissue of SHR and the level of miR-1significantly increased while the level of miR-133 significantly decreased.