高精油互叶白千层组培产业化技术研究

Commercialization of Tissue Cultivation for Melaleuca altennifolia

高精油互叶白千层优良无性系继代苗经多代增殖扩繁后，易出现基部褐化死亡现象，部分无性系生根率低，严重阻碍了组培苗产业化生产应用。以花宝1号、B5、MS 为基本培养基，开展大量元素不同浓度、维生素组合、生长调节剂对比试验。结果表明：3．0 g·L^－1花宝1号与 VH、Vc、VB2的组合能有效地抑制继代苗褐化死亡；继代培养基中添加 GA0．4～0．6 mg·L^－1，有效芽苗数多，且芽苗粗壮、长势整齐；不同优良无性系在2．0 g·L^－1花宝1号生根培养基均获得了较高生根率，平均生根率为95％。花宝1号能有效提高互叶白千层组培苗产业化效率。

Plantlets from the elite,high essential oil containing Melaleuca altennifolia tended to turn brown and die after generations of multiplication.In addition,the low rooting rate prohibited the commercialization of the tissue cultivation.Using Hyponex No.1,B5 and MS as the basis,media formulated with varied concentrations of substrates,vitamins and growth regulators were used in an attempt to improve the cultivation.The results showed that （a）3.0 g·L^-1 of Hyponex No.1 in combination with VH,Vc and VB2 effectively inhibited the browning and death of the plantlets;（b）a supplementation of 0.4 -0.6 mg·L^-1 GA in the subculture medium increased the number of effective seedlings,and encouraged a strong and uniform growth of the seedlings;and,（c）the addition of Hyponex No.1 at a rate of 2.0 g · L^-1 in the rooting medium raised the average rooting rate for different individuals to 95%.