枸杞多糖对辐射雄性大鼠睾丸细胞凋亡的影响（英文）

Effects of Lycium Barbarum Polysaccharide on Testicular Cell Apoptosis Induced by Low Dose Ionizing Radiation in Male Rats

目的观察枸杞多糖(Lycium barbarum polysaccharide,LBP)对低剂量电离辐射所致雄性大鼠睾丸细胞凋亡的影响。方法 Wistar雄性大鼠84只,随机分为空白对照组、辐射组对照组1、辐射对照组2、辐射对照组3、LBP+辐射组1、LBP+辐射组2、LBP+辐射组3共7组,每组12只,LBP组在每次辐射前给与10 mg/kg LBP干预、其余各组以等量生理盐水灌胃;除空白对照组外,各组进行60Coγ射线局部照射(周一至周五),连续4w,总剂量2.3 Gy/capita。分别于停止照射后1d、7d、14d处死大鼠,。检测脏器系数、精子计数和活力,用RT-PCR,Weston-blot法检测P53和HSP70的m RNA的相对表达量以及蛋白表达。结果 LBP+辐射组1,2,3的睾丸系数较各自辐射对照组增加,以LBP+辐射组3最为明显(P<0.01),但对附性腺器官系数没有明显影响;与辐射对照组1,2,3相比,LBP+辐射组1,2,3的大鼠各组的精子计数和活力明显提高(P<0.01),RT-PCR,Weston-blot法显示LBP+辐射组3的P53m RNA的相对表达量和蛋白表达较辐射对照组3明显降低,而HSP70m RNA的相对表达量和蛋白表达则明显升高(P<0.01);结论 LBP对低剂量电离辐射造成大鼠生殖系统的损伤有一定保护和修复作用。该作用可能是通过减少睾丸细胞的凋亡途径而实现的。

Objective To observe the effects of lycium barbarum polysaccharide （LBP） on testicular cell apoptosis induced by low dose ionizing radiation in male rats. Methods Eighty four Wistar male rats were randomly divided into seven groups： normal control group; irradiation control groups 1, 2 and 3; LBP ＋ irradiation groups 1, 2 and 3. The rats in LBP groups were given 10 mg/kg LBP by gavage before irradiation, while the rats in other groups received saline. The testes of all rats except those in normal control group were exposed to ^60Coγ-ray irradiation locally from Monday to Friday for 4w and the total dose was 2.3 Gy/capita. The rats were sacrificed at days 1, 7, 14, respectively. The testicular index, sperm count and mobility were measured. The expressions of P53 and HSP70 mRNA and protein were assessed using RT-PCR and Western blot methods. Results Both the sperm count and mobility were improved significantly in LBP＋irradiation groups 1, 2 and 3 as compared to corresponding irradiation control groups （P〈0.01）. The testicular index was increased significantly in LBP ＋ irradiation groups, especially in group 3 compared to their corresponding irradiation control groups （P〈0.01）. The P53 mRNA and protein expressions were lower and HSP70 mRNA and protein expressions higher in the LBP ＋ irradiation group 3 than in the irradiation control group 3 （P〈0.01）. Conclusion LBP can reduce the apoptosis of testicular cells induced by, low dose ionizing radiation in male rats and the mechanism was associated with regulation of P53 and HSP70 expression.