烟酰胺超载对大鼠血浆内甲基化反应水平的影响

Effect of Excess Nicotinamide on Plasma Methylation Reaction Levels in Rat

利用长期大鼠高烟酰胺喂养模型,观察烟酰胺超载对血浆内甲基化反应指标的影响。结果发现,高烟酰胺摄入将伴随高甲基供体消耗,并引起血中甜菜碱水平明显降低,直接证明过量烟酰胺会导致甲基库耗竭。这表明过量的烟酰胺可能通过甲基耗竭诱导氧化性线粒体和DNA损伤及DNA甲基化样式的改变。

Long-term high nicotinamide feeding test was performed to investigate the effect of excess nicotinamide on plasma methylation reaction levels. The results shown high nicotinamide intake may lead to a depletion of labile methyl groups. Nicotinamide overload significantly lower plasma betaine levels which suggested the deletion of methyl groups. Therefore, the present study demonstrated that excess nicotinamide may trigger oxidative damage and abnormal DNA methylation patterns due to promote the generation of ROS and its depletion of the methyl donor pool.