趋化因子CXCL16在乳腺癌中的表达及其意义

Expression of chemokine CXCL16 and its significance in breast cancer

目的探讨趋化因子CXCL16在乳腺癌中的表达及其意义。方法收集2012年3月至2013年12月深圳市妇幼保健院94例浸润性乳腺癌、20例乳腺原位癌以及20例乳腺良性病变组织标本,应用免疫组织化学方法检测CXCL16的表达情况,并分析其表达与乳腺癌患者年龄、肿瘤大小、组织学分级、TNM分期及淋巴结转移等临床病理特征之间的关系。数据分析采用χ2检验及秩和检验。结果在乳腺浸润性癌、原位癌和良性病变组织3组之间,CXCL16蛋白表达的差异具有统计学意义(χ2=22.058,P=0.000)。浸润性乳腺癌中CXCL16蛋白的表达均高于原位癌和良性病变组织(P=0.007、0.000)。并且,在不同肿瘤大小、不同TNM分期和有无区域淋巴结转移的浸润性乳腺癌之间,CXCL16蛋白表达的差异均有统计学意义(Z=-2.013、-2.166、-2.222,P=0.035、0.030、0.026),而在不同年龄及组织学分级者之间,其差异均无统计学意义(Z=-0.418,P=0.676;χ2=0.011,P=0.995)。结论 CXCL16在乳腺癌的发生、发展过程中可能起一定的作用。

Objective To explore the expression of chemokine CXCL16 in human breast cancer and its clinical significance. Methods The tissue samples were collected from 94 cases of invasive breast cancer, 20 cases of carcinoma in situ and 20 cases of breast benign lesions in Shenzhen Maternity and Child Healthcare Hospital from March 2012 to December 2013. The expressions of CXCL16 protein in samples were detected by immunohistochemical method. The relationships between the expression of CXCL16 and the clinicopathological characteristics of breast cancer patients including patients＇ age, tumor size, histological grade, TNM stage and lymph node metastasis status were also analyzed. The data was processed using X2 test and rank sum test. Results The expression of CXCL16 protein was statistically different among invasive breast cancer group, carcinoma in situ group and benign lesion group （ X2 = 22. 058, P = 0. 000 ）. CXCL16 protein expression in invasive breast cancer was significantly higher than that in carcinoma in situ or in benign lesions （ P = 0. 007, 0. 000）. CXCL16 protein expression showed a statistical difference among invasive breast cancer patients with different tumor sizes, TNM stages and with or without lymph node metastasis （ Z = -2. 013, -2. 166, -2. 222 ; P=0. 035,0. 030,0. 026）, but no significant difference was observed between different age groups and between the patients with different histological grades （Z=-0.418, P=0.676; X2 =0.011,P=0.995）. Conclusion CXCL16 may play a certain role in the development and metastasis of breast cancer.