白念珠菌热休克蛋白90的表达及其与耐药性的关系

Expression of Candida albicans heat shock protein 90 and its relationship with drug resistance

目的检测白念珠菌热休克蛋白90(Hsp90)的表达水平,探讨Hsp90和白念珠菌耐药性的关系。方法采用荧光定量聚合酶链反应(RT-PCR)方法分别检测10株对伊曲康唑敏感白念珠菌和10株对伊曲康唑耐药白念珠菌的Hsp90的表达情况,并分析其与白念珠菌耐药性的关系。结果对伊曲康唑耐药白念珠菌中Hsp90表达量(1.5±0.9)×105明显高于对伊曲康唑敏感白念珠菌中Hsp90表达量(0.7±0.3)×105,且差异有统计学意义(P<0.05)。结论 Hsp90可能在白念珠菌耐药性的产生和维持过程中起着重要作用,Hsp90的过度表达可能是白念珠菌耐药的重要机制之一。

Objective To investigate the expression of heat shock protein 90 （Hsp90）in Candida albicans and its relationship with drug resistance .Methods The expression levels of Hsp90 of 20 strains of Candida albi‐cans （among them ,the 10 strains resistant to itraconazole ,the other 10 strains sensitive to itraconazole） were de‐tected by using fluorescent quantitative reverse transcription‐polymerase chain reaction （RT‐PCR）. Results Ex‐pression levels of Hsp90 of itraconazole resistant Candida albicans （1.5 ± 0.9） × 105 were significantly higher than that of itraconazole sensitive Candida albicans （0.7 ± 0.3）× 105 ,being of significant differences（ P 〈0. 05） .Con‐clusion Hsp90 may play an important role in the formation and maintenance of Candida albicans drug resistance . Overexpression of Hsp90 may be an important mechanism for drug resistance of Candida albicans.