摘要
为了研究芒果CCR基因在芒果对芒果树抗性的功能。采用传统的RT-PCR和RACE方法从芒果的枝梢中克隆得到了1个参与木质素生物合成的肉桂酰辅酶-A还原酶基因(CCR)的全长c DNA序列,进而对得到的序列采用TMHMM、DNAMAN等软件进行生物信息学分析,发现芒果CCR基因包含编码区、3'和5'非翻译区的长度为1 292 bp的c DNA序列,编码305个氨基酸;对跨膜结构域进行了预测,发现该基因无跨膜结构域;蛋白二级结构元件以无规则卷曲和β-螺旋为主;聚类分析发现,该基因与美洲山杨木、油茶等植物的亲缘关系较近,而与百合、橡胶树等亲缘关系较远。从芒果中克隆得到了一个CCR基因,并对其生物信息学进行了分析,为后续转基因工作打下了基础。
In order to study the resistance function of mango of a cinnamoy-Co A reducase（CCR） gene.CCR was cloned from mango with 3＇RACE and 5＇RACE method.The characteristics of c DNA sequence was analyzed by software such as DNAMAN,TMHMM.The bioinformatics analysis on the obtained sequence was showed：CCR gene of mango contains coding region,3＇and 5＇untranslated region of length c DNA sequence of 1 292 bp,encoding 305 amino acid.The gene without transmembrance by transmembrance domains predicted.The secondary structure of CCR protein had random coil and beta helix.It was found that the gene encoding for the protein had close relationship with American aspen wood,oil-tea camellia,other distantly relationship with lily,and rubber tree through phylogenetic analysis.The CCR gene was cloned from mango and the bioinformatics analysis,laid the groundwork for the follow-up work of transgenic.
出处
《华北农学报》
CSCD
北大核心
2014年第B12期16-19,共4页
Acta Agriculturae Boreali-Sinica
基金
农业部非营利性科研机构改革启动经费(CATAS
PZS-201225
CATAS-TCGRI
1630032013003
1630032014025)
