不同月龄乌珠穆沁羊骨骼肌中Myostatin表达量的分析

Analysis of the Myostatin Expression in the Skeletal Muscle of Ujumqin Sheep of Different Ages

为研究自然放牧乌珠穆沁羊成长过程中骨骼肌内MSTN基因及其蛋白表达量的变化。分别采用实时荧光定量和ELISA技术测定乌珠穆沁羊生长过程中骨骼肌MSTN基因相对表达量和蛋白表达量。MSTN基因相对表达量为6月龄〉3月龄〉9月龄〉1月龄〉12月龄〉18月龄,6月龄的基因表达量最高,与其他月龄之间相比有显著差异（P〈0.05）。1,3,9,12月龄之间基因表达量无显著差异（P〉0.05）。MSTN蛋白表达量为1月龄〈3月龄〈6月龄〈9月龄〈12月龄〈18月龄,1,3,6月龄的表达量之间有显著差异（P〈0.05）,9,12,18月龄的表达量之间无显著差异（P〉0.05）。MSTN基因相对表达水平1~6月龄之间有上升趋势之后又下降。MSTN蛋白表达水平一直为上升趋势。

The Myostatin（MSTN） gene and protein expressions in the skeletal muscle of Ujumqin Sheep were studied during its growth.RT-PCR and ELISA were applied to detect the relative expression level of MSTN gene and protein,respectively,in the skeletal muscle of Ujumqin sheep at the age of 1,3,6,9,12 and 18 months.The relative MSTN gene expression level was in the descending sequence of 6〉 3〉 9〉 1 〉12〉 18-month old Ujumqin sheep.The MSTN gene expression was highest at the age of 6-month,and was significantly different from that of 1,3,9,12 and 18-month（P 〈0.05）.The detected MSTN gene expression was not significantly different among samples collected from 1,3,9 and 12-month old sheep（P 〉0.05）.The protein expression level was in the ascending sequence of 1 〈3 〈6 〈9 〈12〈 18-month old sheep.Samples from 1,3 and 6-month old（P〈 0.05）,but not 9,12 and 18（P 〉0.05）,sheep were significantly varied.The relative gene expression level of MSTN showed an increasing trend before 6-month old and decreased afterwards.The expression level of MSTN protein was increasing during the whole period.