FT/TFL类基因在山葡萄雌雄花中的表达分析

FT/TFL Expression Pattern in Male and Female Floral of Vitis amurensis Rupr.

为了研究FT/TFL类基因山葡萄雌花和雄花中的表达模式,以山葡萄3个雄花品系和3个雌花品系为材料,根据Gen Bank上已经发表的3个葡萄FT/TFL类基因设计引物,利用实时荧光定量PCR技术研究Vv FT、Vv TFL1A和Vv TFL1B这3个基因在山葡萄雌花和雄花中的表达。3个基因在山葡萄雌花和雄花中都有表达。Vv FT基因在雌花中的表达量显著高于雄花。而Vv TFL1A和Vv TFL1B这2个基因在雄花中的表达量略高于雌花。Vv FT基因的表达高峰出现在花序刚开始展露期(5月14日);而Vv TFL1A和Vv TFL1B表达高峰出现在芽体膨大期(4月30日)。结果表明,这两类基因在山葡萄花发育过程中作用时间不同。FT类基因可能参与花的前期调控,而TFL类基因在花器官发育中起作用。

To study FT / TFL genes expression pattern in female and male floral of Vitis amurensis Rupr.,three male and three female strains were used to study the expression of three FT / TFL genes（Vv FT,Vv TFL1 A and Vv TFL1B） by Real-time PCR technology.The three genes expressed not only in female but also in male floral.The relative expression of Vv FT gene in female were significantly higher than in male floral,while the relative expression of Vv TFL1 A and Vv TFL1 B genes in male flowers slightly higher than in female floral.The peak value of Vv FT gene expression appeared at inflorescence show stage（May 14）;while Vv TFL1 A and Vv TFL1 B expression appeared one peak value at bud intumescence stage（April 30）.It was suggested that FT genes may be involved in regulation of early flowers,and TFL genes played a role in floral organ development.