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植物基因功能研究新方法 被引量:1

New Method for Studying Plant Gene Function
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摘要 TALEN(Transcription activator-like effector nuclease)和CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats,CRISPR.CRISPR-associated,Cas)是近年来发现的基因功能研究的新方法,且已经成功地在动物、植物及微生物等多个领域得到成功应用。它们可以对DNA双链进行切割,从而启动细胞内的DNA修复机制,以此来实现对靶位点的基因修饰。相较于传统方法,它们具有特异性高、操作简便及打靶效率高等特点。主要从结构、功能及作用机制等方面阐述这2种新基因功能的研究方法。 TALEN(Transcription activator-like effector nuclease) CRISPR / Cas system(Clustered regularly interspaced short palindromic repeats,CRISPR.CRISPR-associated,Cas) have recently emerged as new technique for targeted genome modifications.They have been successfully applied in many fields such as animals,plants and microorganism.They can generate a double-strand break(DSB).Repair of these DSBs by intracellular DNA repair mechanisms enables to achieve the target gene modification.Compared with traditional method,TALEN and CRISPR / Cas system were stronger specificity,manipulated more easily and higher targeting efficiency.Here we report the construction,function and mechanism of the TALEN and CRISPR / Cas system.
出处 《华北农学报》 CSCD 北大核心 2014年第B12期109-113,共5页 Acta Agriculturae Boreali-Sinica
基金 公益性行业(农业)科研专项(201303102)
关键词 基因功能 TALEN CRISPR/Cas系统 Gene function TALEN CRISPR/Cas system
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  • 1Li T, Huang S, Jiang W Z, et al. Hybrid proteins composed of TAL effectors and Fok I DNA-cleavage domain [ J ]. Nucleic Acids Research,2011,39(1) :359 -372.
  • 2Moscou M J, Bogdanove A J. A simple cipher governs DNA recognition by TAL effectors [ J ]. Science, 2009, 326(5959) :1501.
  • 3Boch J,Scholze H,Schornack S,et al. Breaking the code of DNA binding specificity of TAL-type III effectors [J]. Science ,2009,326( 5959 ) : 1509 - 1512.
  • 4Tesson L, Usal C, M6noret S,et al. Knockout rats genera- ted by embryo microinjection of TALENs[J]. Nature Bio- technology ,2011,29 ( 8 ) :695 - 696.
  • 5Lei Y,Guo X,Liu Y,et al. Efficient targeted gene disrup- tion in Xenopus embryos using engineered transcription activator-like effector nucleases ( TALENs ) [ J ]. Proceed- ings of the National Academy of Sciences of the United States of America,2012,109(43 ) : 17484 - 17489.
  • 6Huang P, Xiao A, Zhou M, et al. Heritable gene targeting in zebrafish using customized TALENs [ J ]. Nature Bio- technology, 2011,29 ( 8 ) : 699 - 700.
  • 7Mahfouz M M,Li L,Piatek M,et al. Targeted transcrip- tional repression using a chimeric TALE-SRDX repressor protein[ J]. Plant Molecular Biology ,2012,78 ( 3 ) :311 -321.
  • 8Li T, Liu B, Spalding M H ,et al. High-efficiency TALEN- based gene editing produces disease-resistant rice [ J ]. Nature Biotechnology, 2012,30 ( 5 ) : 390 - 392.
  • 9Jiyong Liu,Changqing Li,Zhongsheng Yu,Peng Huang,Honggang Wu,Chuanxian Wei,Nannan Zhu,Yan Shen,Yixu Chen,Bo Zhang,Wu-Min Deng,Renjie Jiao.Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy[J].Journal of Genetics and Genomics,2012,39(5):209-215. 被引量:44
  • 10Wood A J, Lo T W, Zeitler B,et al. Targeted genome edi- ting across species using ZFNs and TALENs [ J ]. Sci- ence, 2011,333 ( 640 ) : 307.

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