小花棘豆黄酮对绵羊瘤胃微生物数量的影响

Effects of Flavonoid of Oxytropis glabra DC on Microbial Number in Rumen of Sheep

探讨小花棘豆黄酮对绵羊瘤胃微生物数量的影响,为小花棘豆的综合利用提供参考。将12只和田羊随机分为4组,即对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组。试验组分别按10,30,50 mg/kg的剂量饲喂小花棘豆黄酮,试验持续35 d。试验前及试验后每隔7 d取瘤胃液,检测瘤胃液中微生物数量的变化。结果显示,与对照相比,试验羊瘤胃液中总细菌数均有不同程度的升高,其中从第21天开始试验Ⅰ组和试验Ⅱ组瘤胃液总细菌数均显著高于对照(P<0.05),但试验Ⅲ组与对照差异不显著(P>0.05);所有试验羊瘤胃液中厌氧真菌数和瘤胃原虫数均无明显变化,试验Ⅰ组和试验Ⅱ组瘤胃液纤维素降解菌数量无明显变化,试验Ⅲ组可降低白色瘤胃球和黄化瘤胃球菌数,但与对照差异不显著(P>0.05)。结果表明,在日粮中适量添加小花棘豆黄酮对绵羊瘤胃微生物数量无不良影响。

12 Hetian sheep were divided into 4 groups（the control group and experimental groupⅠ,Ⅱ,Ⅲ） to investigate the effects of flavonoid of Oxytropis glabra DC feeding on microbial number in rumen,reveal the utilization of O.glabra DC in future.The dried plant of the O.glabra DC was comminuted,and three experimental groups were fed with flavonoid of O.glabra DC at 10,30,50 mg / kg everyday respectively during the test period.The sheep consumed the forages freely until typical symptoms were observed.The test period was 35 d.Before challenge test and at intervals of 7 days in this test,rumen liquid was collected and the microbial number were test.The results demonstrated that the number of total bacteria in experimental groupⅠ and experimental group Ⅱ were significantly higher than normal control group from 21 d（P 〈0.05）,and the number of total bacteria in experimental group Ⅲwere not obvious in brainstem（P〉 0.05）.The correlations between flavonoid of O.glabra DC and number of Anaerobic fungi and protozoa in rumen were poor.The number of Fiber degrading bacteria in rumen of experimental groupⅠ and experimental groupⅡ had no significant changes,The number of Ruminncnccus albus and Ruminococcus flavefaciens in experimental group Ⅲ were lower than normal control group,but had not obvious in brainstem（P 〉0.05）.The results showed that the amount dose of flavonoid of O.glabra DC had no bad effects on microbial number in sheep.