差异NaCl处理时间对青稞和大麦生理影响的比较

Comparation of Different Physiologcal Responses Between Hulless Barley and Barley under Different NaCl Stress Periods

为了确定在较长期Na Cl胁迫条件下,青稞和大麦2种抗逆性较强的禾本科作物的耐盐性表型差异,并为应用比较基因组学技术进一步开发利用禾本科作物抗逆遗传资源打下基础。以1.0%及0.5%Na Cl溶液（w/V）处理的大麦和青稞种子苗为材料,检测分析了这2种植物在Na Cl胁迫1~5 d（4 d）条件下的营养生长状况及其对外界胁迫条件的生理响应表型。结果表明,相同Na Cl胁迫条件下,青稞种子的萌发率显著高于大麦,而且在较长期的Na Cl胁迫条件下青稞种子苗根和叶的生长状况均好于同期大麦。种子苗耐盐生理指标测定结果显示,在相同Na Cl胁迫条件下青稞组织内的丙二醛（Malonyldialdehyde,MDA）含量极显著低于大麦,而脯氨酸、可溶性糖含量以及超氧化物歧化酶（Superoxide dismutase,SOD）比活力则高于大麦,上述结果显示：青稞幼苗比大麦有更好的耐盐性。

To analysis the different phenotypes of hulless barley and barley under Na Cl stress,and also to mine the genetic resources of these two traditional cereal crops with strong resisitance to abiotic-stresses,the relative growth rate of seedings,roots and leaves,as well as some key physiological figures closely related to salt stress responses were examined in hulless barley and barley,which were planted under different treating periods of 1.0% or0.5% Na Cl.The result indicated that,the germination rate of hulless barley seeds is significantly higher than that of barley under the same stress condition triggered by Na Cl.As well as the relative growth rates of roots and leaves of hulless barley seedings are higher than those of barley.Subsequence data analysis of physiological phenotypes revealed that the content of MDA in leaves is significant lower in hulless barley,while the content of free proline,soluble saccharides and the SOD activity in leaves are significantly higher in hulless barley under the same stress condition.As a conclusion,we believe that the hulless barley harbors a better intracellular resistance to Na Cl-stress than barley.