芍药甘草汤功能与药效组分对应性研究

Correlation between active components alignment and medicinal function of Peony and Licorice decoction

目的研究经方芍药甘草汤9种药效组分(氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷、甘草酸、甘草苷、甘草素、异甘草苷和异甘草素)的变化规律,探讨芍药甘草汤功能与药效组分的对应性。方法以经方芍药甘草汤(《伤寒论》方)为载体,用5个不同配伍组分的芍药甘草汤作为对照,采用高效液相二极管陈列检测法测定含量。结果不同配伍的芍药甘草汤9种药效组分为(mg/ml):《伤寒论》方为1.92、0.90、3.51、0.53、8.35、3.16、0.77、0.77和0.15;《传信适用方》为0.92、0.45、1.62、0.37、0.78、0.34、0.06、0.10和0.01;《朱氏集验方》一为1.59、0.82、3.28、0.49、3.15、1.10、0.27、0.44和0.08;《朱氏集验方》二为1.14、0.52、2.12、0.41、2.92、0.97、0.22、0.36、0.06;《魏氏家藏方》为1.38、0.59、2.70、0.43、2.40、0.35、0.06、0.10和0.01;《医门八法》方为1.52、0.67、2.91、0.45、3.00、1.07、0.24、0.42和0.07。结论 1.9种药效组分变化规律为:《伤寒论》方>《朱氏集验方》一>《医门八法》方;《朱氏集验方》二中的药效组分氧化芍药苷、芍药内酯苷、芍药苷和苯甲酰芍药苷含量<《魏氏家藏方》,而甘草酸、甘草苷、甘草素、异甘草苷和异甘草素含量>《魏氏家藏方》;《传信适用方》方中9种药效组分含量最低。2.配伍不同,药效组分总量和比例也不同,临床疗效也各异:《伤寒论》方药效组分总量为20.06 mg/ml,用于止痛;《朱氏集验方》一药效组分总量为11.216 mg/ml,用于消渴;《朱氏集验方》二药效组分总量为8.72 mg/ml,用于治脚弱无力;《传信适用方》药效组分总量为4.65 mg/ml,用于治湿气腿脚赤肿疼痛;《魏氏家藏方》药效组分总量为8.02 mg/ml,用于治湿热脚气;《医门八法》方药效组分总量为10.35 mg/ml,用于治胃气痛。

Objective To study the change regulation of nine compounds（oxypaeoniflorin,albiflorin,paeoniflorin,benzoylpaeoniflorin,glycyrrhizic acid,liquiritin,isoliquiritin,liquiritigenin,and isoliquiritigenin） in Peony and Licorice decoction,and analyze the correlation between therapeutical effects and active components alignment. Methods Classical prescription Peony and Licorice decoction（Shanghanlun）were taken as the carrier,five different combinations of Peony and Licorice decoction were used as the control,and the content was determined by HPLC-DAD. Results The contents of nine compounds of different combinations of Peony and Licorice decoction was（mg/ml）： Shanghanlun 1.92,0.90,3.51,0.53,8.35,3.16,0.77,0.77 and 0.15; Chuanxinshiyongfang0.92,0.45,1.62,0.37,0.78,0.34,0.06,0.10 and 0.01;Zhushijiyanfang A 1.59,0.82,3.28,0.49,3.15,1.10,0.27,0.44 and 0.08;Zhushijiyan-fang B 1.14,0.52,2.12,0.41,2.92,0.97,0.22,0.36 and 0.06;Weishijiacangfang 1.38,0.59,2.70,0.43,2.40,0.35,0.06,0.10 and 0.01;Yi-menbafang 1.52,0.67,2.91,0.45,3.00,1.07,0.24,0.42 and 0.07. Conclusion 1. The change regulation of the nice compounds was Shang-hanlun〉 Zhushi-jiyanfang A 〉Yimenbafang;oxypaeoniflorin, albiflorin, paeoniflorin and benzoylpaeoniflorin in Zhushijiyanfang B〈 Weishijiacang-fang, gly-cyrrhizic acid,liquiritin,isoliquiritin,liquiritigenin and isoliquiritigenin in Zhushijiyanfang B〉 Weishijia cangfang. chuanxinshiy-ongfang has the the lowest content of the nine compounds. 2. Total contents and proportion of the nine compounds are different due to different compatibility.The clinical curative effect is also different. Total contents of the nine compounds in Shanghanlun were 20.06 mg/ml which is used to relieve pain in the legs;in Zhushijiyanfang A was8.72 mg/ml which is used to relieve pain caused by diabetes;in Zhushijiyanfang B was 8.72 mg/ml which is used to treat foot weakness;in Chuanxinshiyong-fang was 4.65 mg/ml which is used to treat wet beriberi;in Weishijiacangfang was 8.02 mg/ml which is used to treat wet beriberi;in Yimenbafang was 10.35 mg/ml which is used to treat peratodynia.