肺表面活性物质对哮喘大鼠氧化-抗氧化系统的影响

EFFECT OF PULMONARY SURFACTANT ON OXYGENATION/ANTAGONIZING OXYGENATION SYSTEM OF RAT ASTHMA MODEL

通过肺表面活性物质 (PS)对大鼠哮喘模型的干预 ,观察哮喘大鼠体内氧化 -抗氧化系统的变化 ,探讨PS治疗哮喘的机理。方法 :采用卵蛋白致大鼠过敏性哮喘的实验模型 ,设立正常对照组、哮喘对照组、PS治疗组 ,分别检测各组大鼠血清中超氧化物歧化酶 (SOD)的活力及丙二醛 (MDA)含量。结果 :哮喘对照组与正常对照组相比 ,血清SOD活力降低 (P <0 .0 1) ,MDA含量升高 (P <0 .0 0 1) ;PS治疗组血清SOD活力高于哮喘对照组 (P <0 .0 5 ) ,MDA含量低于哮喘对照组 (P <0 .0 5 ) ;而PS治疗组与正常对照组相比 ,SOD活力及MDA含量均无显著性差异 (P >0 .0 5 )。结论 :哮喘大鼠发作期体内氧自由基增多、抗氧化能力下降 ,使用PS治疗后 ,氧自由基减少 ,氧化 -抗氧化系统的失衡有所纠正 ,提示PS在哮喘治疗方面重要的应用前景。

Objective:This study was due to investigate the effect of pulmonary surfactant (PS) on oxygen free radicals of asthma rat model and to investigate the therapeutic mechanism of PS.Methods:Adopt the asthma rat model sensitized and provoked with ovalbumin and set up three groups (normal control group,asthma control group and PS group).Serum of SOD and MDA were measured respectively.Results:SOD levels of serum in asthma control group were significantly decreased compared with those in normal control group ( P <0.01),while MDA levels of serum were significantly elevated ( P <0.001).SOD levels of serum in PS group were higher than those in asthma control group ( P <0.05),on the contrary,MDA levels of serum were lower than those in asthma control group ( P <0.05).There were no remarkable difference on both SOD levels and MDA levels between PS group and normal control group ( P >0.05).Conclusions:The level of oxygen free radicals of asthma rat model in the attacking duration was increased and the capacity of antagonizing oxygenation was reduced.After administration of surfactant oxygen free radicals was reduced and the balance of oxygenation and antagonizing oxygenation was restored somewhat.It is suggested that PS has important applied future in the treatment of asthma.