摘要
研究糖尿病大鼠肾脏血管紧张素Ⅱ 2型受体及Ⅳ型胶原的mRNA表达。方法 :大鼠随机分成两组 :单肾切组、糖尿病组。实验第 8周 ,应用RT -PCR检测大鼠肾皮质血管紧张素Ⅱ 2型受体 (AT2 )及Ⅳ型胶原mRNA表达。同时用放免法检测大鼠肾皮质血管紧张素Ⅱ含量。结果 :糖尿病组大鼠尿蛋白排泄率 (t=9.32 ,P <0 .0 1)、肾皮质血管紧张素Ⅱ水平 (t=2 .2 7,P <0 .0 5 )及Ⅳ型胶原mRNA表达 (t =3.71,P <0 .0 1)均较单肾切组明显升高 ;而AT2 的mRNA水平却下降 (t=2 .35 ,P <0 .0 5 )。结论 :糖尿病肾组织AT2 受体mRNA表达的下降及Ⅳ型胶原表达的增加参与了糖尿病肾病的发生、发展。
Objective:To investigate mRNA expression of the type 2 angiotensin Ⅱ receptor and collagen Ⅳ in diabetic rats'kidney.Methods:The rats were randomly divided into following groups: uninephrectomized (group C) and diabetic rats (group D). At 8 weeks' duration of study,mRNA expressions of the type 2 angiotensin Ⅱ receptor (AT 2) and collagen Ⅳ in rats'renal cortex were measured by quantitative reverse transcription polymerase chain reaction (RT-PCR), respectively. In addition, angiotensin Ⅱ level in renal cortex was determined by the radioimmunoassay.Results:In group D, urine protein excretion (t=9.32, P <0.01 ), angiotensin Ⅱ level (t=2.27, P <0.05) and mRNA expression of collagen Ⅳ (t=3.71, P <0.01 ) in rats'renal cortex were increased obviously in contrast to group C, however mRNA expressions of AT 2 in group D showed a significant decrease (t=2.35, P < 0.05 ).Conclusions: The decreased mRNA expressions of AT 2 and elevated collagen Ⅳ expression might play an important pathogenetic role in the initiation and progression of diabetic nephropathy.
出处
《中国现代医学杂志》
CAS
CSCD
2002年第15期17-19,共3页
China Journal of Modern Medicine
基金
浙江省教委部分资助 (基金编号 2 0 0 0 0 666)
