摘要
目的:筛选胃癌(gastric cancer,GC)转移相关的差异表达微小RNA(micro RNA,miR NA),探讨miR NA-210与GC转移的可能机制.方法:应用mi RNA表达谱芯片筛选GC高转移细胞株RF48及低转移细胞株RF1的差异表达mi RNAs,RT-PCR检测mi RNA-210在7种GC细胞株中的表达情况,利用mi RWalk软件获得mi RNA-210的靶基因,通过David在线软件(包括GO分析及KEGG通路预测)分析mi R-210靶基因的可能生物学功能.结果:与RF1相比,mi RNA芯片在RF48细胞中共筛选出21个表达上调和15个表达下调的mi RNA,其中mi RNA-210在高转移细胞株RF48中的表达增高;RT-PCR结果显示m i R N A-210在高转移G C细胞株中表达增高(P<0.05);mi RWalk共获得155个mi RNA-210靶基因;GO分析及KEGG pathway分析得到miR NA-210靶基因功能,这些靶基因参与了肿瘤的发生、发展及转移.结论:利用mi RNA芯片技术获得了GC转移相关mi RNA表达谱;mi RNA-210的异常表达可能与GC的转移有关,为GC转移的早期诊断及发现新的治疗靶点奠定了基础.
AIM: To screen microRNAs (miRNAs) associated with metastasis of gastric cancer (GC) by miRNA microarray and to explore the possible role of miRNA-210 in GC metastasis by bioinformatics. METHODS: GC cell lines with low (RF1) or high metastatic potential (RF48) were used for miRNA expression profiling using human miRNA microarray. Expression of miRNA-210 in 7 GC cell lines was detected by RT-PCR. MiRNA-210 targets were obtained using miRWalk, and functions of these targets in GC were predicted with David online. RESULTS: Compared with RF1 cells, 21 and 15 miRNAs were up-regulated and down- regulated in RF48 cells, respectively. Expression of miRNA-210 was further validated by realtime quantitative RT-PCR in multiple GC cell lines with different metastatic potential, which showed that miRNA-210 was overexpressed in GC cell lines with high metastatic potential. Bioinformatics analysis suggested that miRNA-210 was related with tumorgenesis and metastasis.CONCLUSION: Screening miRNAs associated with metastasis lays a foundation for identifying early diagnostic markers and new therapeutic targets for GC metastasis. Expression profile of miRNAs associated with metastasis was obtained by miRNA microarray; dysregulated expression of miRNA-210 may be related with GC metastasis, and may serve as an early diagnostic biomarker and new treatment target.
出处
《世界华人消化杂志》
CAS
2015年第1期78-84,共7页
World Chinese Journal of Digestology
基金
国家自然科学基金面上资助项目
No.81172282
国家自然科学基金青年基金资助项目
No.81302151
深圳市海外高层次人才创新创业专项基金资助项目
No.KQCX20130621101141669
深圳市科技研发资金国家和省计划配套基金资助项目
No.GJHS20120621142654087
深圳市肿瘤转化医学重点实验室建设基金资助项目
No.ZDSY20130329101130496
深圳大学科研基金资助面上资助项目
No.80100035905
深圳大学基础研究科研基金资助项目
No.201108
深圳大学科技创新团队基金资助项目
No.T201202~~