miRNA-210在胃癌转移细胞株中的表达及功能预测

Expression of mi RNA-210 in gastric cancer cell lines and its function prediction

目的:筛选胃癌(gastric cancer,GC)转移相关的差异表达微小RNA(micro RNA,miR NA),探讨miR NA-210与GC转移的可能机制.方法:应用mi RNA表达谱芯片筛选GC高转移细胞株RF48及低转移细胞株RF1的差异表达mi RNAs,RT-PCR检测mi RNA-210在7种GC细胞株中的表达情况,利用mi RWalk软件获得mi RNA-210的靶基因,通过David在线软件(包括GO分析及KEGG通路预测)分析mi R-210靶基因的可能生物学功能.结果:与RF1相比,mi RNA芯片在RF48细胞中共筛选出21个表达上调和15个表达下调的mi RNA,其中mi RNA-210在高转移细胞株RF48中的表达增高;RT-PCR结果显示m i R N A-210在高转移G C细胞株中表达增高(P<0.05);mi RWalk共获得155个mi RNA-210靶基因;GO分析及KEGG pathway分析得到miR NA-210靶基因功能,这些靶基因参与了肿瘤的发生、发展及转移.结论:利用mi RNA芯片技术获得了GC转移相关mi RNA表达谱;mi RNA-210的异常表达可能与GC的转移有关,为GC转移的早期诊断及发现新的治疗靶点奠定了基础.

AIM： To screen microRNAs （miRNAs） associated with metastasis of gastric cancer （GC） by miRNA microarray and to explore the possible role of miRNA-210 in GC metastasis by bioinformatics. METHODS： GC cell lines with low （RF1） or high metastatic potential （RF48） were used for miRNA expression profiling using human miRNA microarray. Expression of miRNA-210 in 7 GC cell lines was detected by RT-PCR. MiRNA-210 targets were obtained using miRWalk, and functions of these targets in GC were predicted with David online. RESULTS： Compared with RF1 cells, 21 and 15 miRNAs were up-regulated and down- regulated in RF48 cells, respectively. Expression of miRNA-210 was further validated by realtime quantitative RT-PCR in multiple GC cell lines with different metastatic potential, which showed that miRNA-210 was overexpressed in GC cell lines with high metastatic potential. Bioinformatics analysis suggested that miRNA-210 was related with tumorgenesis and metastasis.CONCLUSION： Screening miRNAs associated with metastasis lays a foundation for identifying early diagnostic markers and new therapeutic targets for GC metastasis. Expression profile of miRNAs associated with metastasis was obtained by miRNA microarray; dysregulated expression of miRNA-210 may be related with GC metastasis, and may serve as an early diagnostic biomarker and new treatment target.