食管鳞状细胞癌组织中CCL26的表达及其临床意义

Expression of CCL26 in esophageal squamous cell carcinoma tissues and its clinical significance

背景与目的:趋化因子在癌症的发生、发展过程中起着重要的作用,但趋化因子CCL26在食管鳞状细胞癌组织中的表达功能报道甚少。本研究旨在探讨趋化因子CCL26在人食管鳞状细胞癌组织及正常食管黏膜组织中的表达水平,分析其与食管鳞状细胞癌相关临床病理指标之间的关系。方法:利用组织微阵列技术制作组织芯片,采用免疫组织化学ABC法分析197例食管鳞状细胞癌组织和相应癌旁正常食管黏膜组织中趋化因子CCL26的表达水平,并对食管鳞状细胞癌中趋化因子CCL26的表达与患者年龄、性别、临床分期、淋巴结转移及5年生存率等临床病理指标的相关性进行分析。结果:1趋化因子CCL26在食管鳞状细胞癌组织及癌旁正常组织中均有表达,在食管鳞状细胞癌组织中趋化因子CCL26的阳性表达率明显高于癌旁正常组,差异有统计学意义(61.8%vs 20.6%,P<0.05);2趋化因子CCL26的表达与淋巴结转移有关,而与患者的年龄、性别、组织学类型、肿瘤细胞分化程度和大体分型等无关(P>0.05);3趋化因子CCL26阳性表达的食管鳞状细胞癌患者5年生存率明显低于趋化因子CCL26阴性表达的食管鳞状细胞癌患者。结论:趋化因子CCL26高表达可能与食管鳞状细胞癌的发生、发展相关,导致5年生存率下降,检测趋化因子CCL26可能为食管鳞状细胞癌预后判断提供依据。

Background and purpose： Chemokines play an important role in cancer occurrence and development. However, little about the function of CCL26 in esophageal squamous cell carcinoma （ESCC） is reported. This study was designed to observe and study the expression of chemokine CCL26 in ESCC tissues and to analyze their association with pathological features of ESCC. Methods： Expressions of chemokine CCL26 in 197 ESCC tissues and their corresponding paraneoplastic normal esophageal tissues were determined by tissue array and immunohistochemistry （IHC） technique, and its correlations to age, gender, lymph nodes metastasis, TNM stage, general classification and 5-year survival rate of ESCC patients were further analyzed. Results： ①CCL26 was expressed in both ESCC and paraneoplastic normal esophageal tissues. The expression of CCL26 in ESCC tissues was signiifcantly higher than that in paraneoplastic normal esophageal tissues （61.8%vs 20.6%, P〈0.05）.②The expression of CCL26 was correlated with lymph node metastasis, but not correlated with age, gender, tumor cell differentiation, and general classiifcation （P〉0.05）.③Survival analysis showed that the abnormal expression of CCL26 was associated with 5-year survival rate of patients with ESCC. The 5-year survival rate of ESCC patients with CCL26 positive expression was obviously lower than that of ESCC patients with CCL26 negative expression. Conclusion： CCL26 upexpression might play an important role in the progression and development of ESCC patients. The high level of CCL26 expression is correlated with lymph node metastasis and poor survival. Detection of CCL26 expression may have important prognostic values in ESCC patients.