摘要
以7个糯玉米品种为材料,测定其籽粒发育过程中淀粉粒粒度分布及淀粉合成相关酶活性的变化,分析两者之间的关系。结果表明,随着籽粒发育,糯玉米淀粉粒平均粒径逐渐增大,可溶性淀粉合成酶(SSS)和淀粉分支酶(SBE)活性呈单峰曲线变化。籽粒发育前期,小淀粉粒(≤7.4μm)所占体积较大;随着籽粒发育,小淀粉粒所占体积减少,大淀粉粒(>7.4μm)所占体积增多;籽粒发育后期,大淀粉粒所占体积较大。相关分析表明,SSS和SBE活性与大淀粉粒体积增大速率和平均粒径增大速率均呈显著或极显著正相关。因此,SSS和SBE是影响糯玉米胚乳淀粉粒粒度分布形成的主要酶,SSS和SBE活性越高,淀粉粒平均粒径越大,大淀粉粒所占体积越多。
In this study, seven varieties of waxy corn（Zea mays var. sinensis） were used to determine starch granule size distribution and starch synthetic enzyme activity and analyze their correlation. The results indicated that with kernel development, mean diameter of starch granule increased, and soluble starch synthase（SSS） and starch-branching enzyme（SBE） activities showed single peak curve. During early period of kernel development, the volume percentage of small starch granule（≤7.4 μm） was large. Then, the volume percentages of small and large starch granules decreased and increased, respectively. During late period, the volume percentage of large starch granule（7.4 μm） was large. Correlation analysis indicated that SSS and SBE activities were positively（highly） signifi cantly correlated with the increasing percentages of large starch granule volume and mean diameter. Therefore, SSS and SBE are the key enzymes to affect starch granule size distribution formation of waxy corn. The higher SSS and SBE activities are, the larger mean diameter and volume percentage of large starch granule are.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第1期88-92,共5页
Plant Physiology Journal
基金
国家自然科学基金(31101100)
山东省科技发展计划(2014GNC111001)
作物生物学国家重点实验室开放课题(2013KF05)
山东省旱地作物水分高效利用科研创新团队
