橙皮苷、柠檬烯及茶多酚清除自由基能力的对比研究

Capacity of Hesperidin,Limonene and Tea Polyphenols on Sequestering Free Radical

目的:研究橙皮苷、柠檬烯及茶多酚对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和羟基自由基(·OH)的清除能力。方法:UV-Vis光谱分析方法测定3种天然产物对DPPH·和·OH自由基的清除作用。结果:3种天然产物在实验浓度范围内,对两种自由基均有清除作用,清除效果随浓度增大而增加。橙皮苷(IC50=0.097,0.011)清除DPPH·和·OH的能力明显强于柠檬烯(IC50=0.0024,0.0004)、茶多酚(IC50=0.0144,0.009)。结论:橙皮苷比茶多酚、柠檬烯清除DPPH·和·OH的能力强。

Objective：To study the capacity of hesperidin,limonene and tea polyphenols on sequestering 1,1diphenyl-2-trinitrobenzene hydrazine（DPPH·）and hydroxyl free radical（·OH）.Methods：UV-Vis spectrum analysis method was used to determinate the three natural products on DPPH· and·OH free radical scavenging.Results：The three natural products in the experimental concentration range have the capacity to remove the free radicals,the removal of effect increases with the concentration.The capacity of hesperidin（IC50=0.097,0.011）is stronger than the limonene（IC50=0.00236,0.0004）,tea polyphenol（IC50=0.0144,0.0009）on DPPH· and ·OH scavenging.Conclusion：The antioxidant capacity of hesperidin is stronger than tea polyphenol and limonene in vitro.